Plexin A3 抗体 (Extracellular)
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- 抗原 See all Plexin A3 (PLXNA3) 抗体
- Plexin A3 (PLXNA3)
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抗原表位
- AA 440-453, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Plexin A3 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (IF), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to Plexin-A3
- 特异性
- Extracellular, N-terminus
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat - identical, human - 12,14 amino acid residues identical
- 产品特性
- Anti-Plexin-A3 (extracellular) Antibody (ABIN7043438, ABIN7045134 and ABIN7045135)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize PLXNA3 from rat, mouse, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3
- 亚型
- IgG
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anti-Plexin A3 (PLXNA3) (AA 950-1150) antibody
PLXNA3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IHC
Negative Control: BLP-PR093
Blocking Peptide: BLP-PR093
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- Plexin A3 (PLXNA3)
- 别名
- PLXNA3 (PLXNA3 产品)
- 背景
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PLXNA3, PLXN3, Semaphorin receptor SEX,Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.
Alternative names: Plexin-A3, PLXNA3, PLXN3, Semaphorin receptor SEX - 基因ID
- 18846
- NCBI登录号
- NM_017514
- UniProt
- P70208
- 途径
- Regulation of Cell Size
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