Plexin A2 抗体 (Extracellular)
Quick Overview for Plexin A2 抗体 (Extracellular) (ABIN7043437)
抗原
See all Plexin A2 (Plxna2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 205-218, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Plexin-A2
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - identical
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产品特性
- Anti-Plexin-A2 (extracellular) Antibody is directed against an epitope of mouse Plexin-A2. Anti-Plexin-A2 (extracellular) Antibody (ABIN7043437, ABIN7045130 and ABIN7045131) can be used in western blot and immunohistrochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting PLXNA2 in living cells. It has been designed to recognize PLXNA2 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RDPESSAMLDYELH, corresponding to amino acid residues 205-218 of mouse PLXNA2
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亚型
- IgG
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anti-Plexin A2 (Plxna2) (AA 1-164) antibody
Plxna2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Plexin A2 (Plxna2) antibodyPlxna2 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Plexin A2 (Plxna2) (Internal Region) antibodyPlxna2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Plexin A2 (Plxna2) (AA 300-560) antibodyPlxna2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Plexin A2 (Plxna2) (AA 434-483) antibodyPlxna2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Plexin A2 (Plxna2) (N-Term) antibodyPlxna2 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Plexin A2 (Plxna2) (N-Term) antibodyPlxna2 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7236264)
Blocking Peptide: (ABIN7236264)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Plexin A2 (Plxna2)
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别名
- PLXNA2
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背景
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PLXNA2, PLXN2, Semaphorin receptor OCT,The Plexin gene family is comprised of at least 9 members in 4 subfamilies. Plexins are widely expressed in neurons but have been also detected in epithelial cells1. Plexins closely interact with a large family of proteins called Semaphorins which control a variety of processes in the CNS including cell migration and axonal growth. Plexins and Semaphorins share a common feature of a conserved 500 amino acid "sema" domain2. The ectodomain of PLXNA2 is an N-terminal seven bladed β-propeller sema domain followed by a cysteine rich PSI (plexin-semaphorin-integrin) domain. PLXNA2 has four N-terminal domains and unlike semaphorins the sema domain of unliganded PLXNA2 does not homodimerize readily.Many of the functions of Class 3 semaphorins (Sema3A-Sema3G) are mediated by Plexin-A2 (PLXNA) and other A-plexins. Sema3 signaling depends on semaphorin interacting with one of the two Nrp co-receptors that associate with PLXNA to generate holoreceptors.PLXNA also interacts with semaphorin 6A (Sema6A). Before binding, the Sema6A ectodomain can be found in a 'face-to-face' homodimer arrangement, similar to the arrangement in Sema3, whereas PLXNA is in an unexpected 'head-on' homodimer arrangement. The structure of the Sema6A-PLXNA signaling complex is a 2:2 heterotetramer3.In prostate cancer TMPRSS:ERG gene fusion causes aberrant expression of the transcription factor ERG and the induction of cell migration and invasion. TMPRSS:ERG upregulates PLXNA expression and elevated PLXNA levels were found in prostate cancer cells. Levels of PLXNA were even higher in metastatic prostate cancer than in localized tumors suggesting PLXNA may play a role in the disease's development.
Alternative names: Plexin-A2, PLXNA2, PLXN2, Semaphorin receptor OCT -
基因ID
- 18845
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NCBI登录号
- NM_025179
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UniProt
- P70207
抗原
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