Peroxisomal Biogenesis Factor 5-Like (PEX5L) (AA 151-165) 抗体
-
- 抗原 See all Peroxisomal Biogenesis Factor 5-Like (PEX5L) 抗体
- Peroxisomal Biogenesis Factor 5-Like (PEX5L)
-
抗原表位
- AA 151-165
-
适用
- 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- 非结合性
-
应用范围
- Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to PEX5L (TRIP8b)
- 特异性
- Intracelular.
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- rabbit - 14, human - 13,15 amino acid residues identical,Mouse - identical
- 产品特性
- Anti-PEX5L (TRIP8b) Antibody is directed against an epitope of the rat PEX5-related protein. Anti-PEX5L (TRIP8b) Antibody (ABIN7043433, ABIN7045126 and ABIN7045127)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PEX5Rp from rat, mouse and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: (C)EKWDDVKFHGDRTSK, corresponding to amino acid residues 151-165 of rat PEX5-related protein
- 亚型
- IgG
-
-
- 应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400
- 说明
-
Negative Control: BLP-PR070
Blocking Peptide: BLP-PR070
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
- 抗原
- Peroxisomal Biogenesis Factor 5-Like (PEX5L)
- 别名
- PEX5L (PEX5L 产品)
- 背景
-
PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein,PEX5R (also called TRIP8b, tetratricopetide repeat-containing Rab8b-interacting protein) is a cytoplasmic protein expressed as a family of alternatively spliced isoforms. PEX5R /TRIP8b isoforms contain a large constant domain preceded by a variable region. The C-terminal half of TRIP8b comprises a tetratricopeptide repeat (TPR) protein binding domain1. Analysis of the Pex5p-like protein revealed the presence of a tetratricopeptide repeat (TPR) domain in its C-terminal half consisting of seven TPR motifs. Pex5R is almost exclusively expressed in brain2.Recent studies provide strong evidence that PEX5R interacts with the carboxyl-terminal region of Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels and regulates their cell-surface expression level and cyclic nucleotide dependence3,4. HCN channels are key modulators of neuronal activity by providing the depolarizing cation current involved in rhythmogenesis, dendritic integration, and synaptic transmission. In contrast, Gosh et al. have shown that human PEX5R can bind to peroxisomal targeting signal-1 (PTS1) ligands5.Furthermore it was shown that enzyme alanine-glyoxylate aminotransferase (AGT) is recognized by PEX5R in the cytoplasm, which allows its subsequent translocation into the peroxisome6. TRIP8b may play a role in both normal neuronal function and in aberrant neuronal excitability associated with neurological diseases7.
Alternative names: PEX5L (TRIP8b), PEX5R, PEX5Rp, PEX5-related protein, PEX5-like protein, Peroxin-5-related protein, PEX2-related protein, PXR2, Peroxisome biogenesis factor 5-like, TPR-containing Rab8b-interacting protein - 基因ID
- 286937
- NCBI登录号
- NM_016559
- UniProt
- Q925N3
- 途径
- Positive Regulation of Peptide Hormone Secretion, Maintenance of Protein Location
-
