OPRL1 抗体 (Intracellular) (Atto 594)
Quick Overview for OPRL1 抗体 (Intracellular) (Atto 594) (ABIN7043400)
抗原
See all OPRL1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 337-352, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594
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特异性
- Intracellular, C-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 13,16 amino acid residues identical
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产品特性
- Anti-Nociceptin Receptor (OPRL1) Antibody (ABIN7043399, ABIN7044893 and ABIN7044894) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot analysis, and has been designed to recognize the nociceptin receptor from mouse, rat and human samples. \nAnti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody (ABIN7043400) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1
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亚型
- IgG
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anti-Opiate Receptor-Like 1 (OPRL1) (AA 1-47) antibody
OPRL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (AA 323-370) antibodyOPRL1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (Middle Region) antibodyOPRL1 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 山羊, 马, 绵羊, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (AA 110-212) antibodyOPRL1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (AA 1-370) antibodyOPRL1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (AA 110-212) antibodyOPRL1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2A11 unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) (C-Term) antibodyOPRL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Opiate Receptor-Like 1 (OPRL1) antibodyOPRL1 适用: 人, 小鼠, 兔 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:60
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7236167)
Blocking Peptide: (ABIN7236167)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- OPRL1 (Opiate Receptor-Like 1 (OPRL1))
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别名
- OPRL1
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背景
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κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor,Nociceptin/orphanin FQ (N/OFQ), a 17 amino acid peptide, is the endogenous agonist of the nociceptin/orphanin FQ peptide (NOP) receptor (previously termed ORL1 receptor) which belongs to the class A (Rhodopsin-like) γ subfamily of G protein-coupled receptors (GPCRs). Like all members the receptor has a common seven-transmembrane helical structure and is coupled predominantly to heterotrimeric Gi/Go proteins. N/OFQ receptor shows a high degree of structural homology with the μ-, δ- and κ-opioid receptors1. The intracellular events triggered by the binding of N/OFQ with the NOP receptor include inhibition of adenylyl cyclase, activation of phospholipase C (PLC) and of K+ channels, inhibition of Ca2+ channels, and activation of mitogen-activated protein-kinases (MAPKs). In neural tissues, the main effect is the inhibition of the release of neurotransmitters (acetylcholine, catecholamines, GABA, glutamate, 5-hydroxytryptamine, tachykinines)2.NOP receptor mRNA is detected in high levels in the cortex, anterior olfactory nucleus, lateral septum, hypothalamus, hippocampus, amygdala, central grey, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus and spinal cord3.Studies with N/OFQ, NOP-selective agonists or antagonists, and receptor- or peptide-deficient mice have shown that the NOP system has important roles in the control of central and peripheral functions including pain, anxiety depression, hyperphagia and obesity, addiction, Parkinson's disease, cognition, food intake, learning and memory, locomotion, cough and micturition reflexes, cardiovascular homeostasis, intestinal motility and immune responses4.
Alternative names: Nociceptin Receptor (OPRL1), Kappa-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor -
基因ID
- 29256
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NCBI登录号
- NM_182647
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UniProt
- P35370
抗原
-
