NUCB2 抗体 (Intracellular)
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Quick Overview for NUCB2 抗体 (Intracellular) (ABIN7043396)
抗原
See all NUCB2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 30-44, Intracellular
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原理
- A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- human -11,Mouse -13,15 amino acid residues identical
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产品特性
- Anti-Nucleobindin-2/Nesfatin-1 Antibody (ABIN7043396, ABIN7044216 and ABIN7044217) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Nucleobindin-2/Nesfatin-1 from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1
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亚型
- IgG
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anti-Nucleobindin 2 (NUCB2) (AA 25-420) antibody
NUCB2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (Middle Region) antibodyNUCB2 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, Pig, 兔, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 322-420) antibodyNUCB2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6H4 unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-106) antibodyNUCB2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-106) antibodyNUCB2 适用: 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-104) antibodyNUCB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 108-246) antibodyNUCB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 182-231) antibodyNUCB2 适用: 人, 大鼠, 犬, 马, Hamster, Bat, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (C-Term) antibodyNUCB2 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 341-390) antibodyNUCB2 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, Pig, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200-1:400
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:1200
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7236159)
Blocking Peptide: (ABIN7236159)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- NUCB2 (Nucleobindin 2 (NUCB2))
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别名
- NUCB2
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背景
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Synonyms: NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109
Description: Nucleobindin contains multiple functional domains including a signal peptide on the N-terminal side, a leucine/isoleucine rich region, a DNA binding domain and a putative nuclear targeting signal. The second half of the protein contains two Ca2+-EF-hand motifs and a leucine zipper motif in the C-terminal region. To date, two nucleobindins have been identified, namely nucleobindin 1 and nucleobindin 2.Nucleobindin 2 (NUCB2) can be found on the plasma membrane and in the cytoplasm. Putative post-translational processing of NUCB2 by the enzyme pro-hormone convertase (PC)-1/3 results in nesfatin-1, nesfatin-2 and nesfatin-3. So far, a biological activity has only been demonstrated for nesfatin-1 and its fragment, nesfatin-124-531.The gene Nesfatin/NUCB2 encoding the protein is expressed in the appetite control hypothalamic nuclei such as the paraventricular nucleus (PVN), the supraoptic nucleus, the lateral hypothalamic area and the zona increta in rats. Nefstain-1 immunoreactivity is also detected in the brain stem nuclei such as the nucleus of the solitary tract and the dorsal nucleus of the vagus.Nesfatin-1 has a substantial anorexigenic effect through the selective reduction of nesfatin/NUCB mRNA expression in the PVN of rats under starved conditions. Refeeding of rats was found to restore Nesfatin-1 reduction. A similar effect was not observed in other hypothalamic nuclei2. In humans, Nesfatin-1 levels in the saliva and serum of epilepsy patients are reduced with the use of anti-epileptic medication, but are still higher than the amounts in normal subjects. In addition, nesfatin-1 levels are decreased in human subjects with type-2 diabetes and it has been hypothesized that the observed decrement might be associated with diabetic hyperphagia3.
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基因ID
- 59295
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NCBI登录号
- NM_005013
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UniProt
- Q9JI85
抗原
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