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- 抗原 See all NUCB2 抗体
- NUCB2 (Nucleobindin 2 (NUCB2))
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抗原表位
- AA 30-44, Intracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NUCB2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- A Rabbit Polyclonal Antibody to Nucleobindin-2/Nesfatin-1
- 特异性
- Intracellular
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- human -11,Mouse -13,15 amino acid residues identical
- 产品特性
- Anti-Nucleobindin-2/Nesfatin-1 Antibody (ABIN7043396, ABIN7044216 and ABIN7044217) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize Nucleobindin-2/Nesfatin-1 from human, mouse, and rat samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DKTKVHNVEPVESAR, corresponding to amino acid residues 30-44 of rat Nucleobindin-2/Nesfatin-1
- 亚型
- IgG
- Top Product
- Discover our top product NUCB2 Primary Antibody
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anti-Nucleobindin 2 (NUCB2) (AA 25-420) antibody
NUCB2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (Middle Region) antibodyNUCB2 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, Pig, 兔, 豚鼠, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 322-420) antibodyNUCB2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6H4 unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-106) antibodyNUCB2 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-104) antibodyNUCB2 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 108-246) antibodyNUCB2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 182-231) antibodyNUCB2 适用: 人, 大鼠, 犬, 马, Bat, 猴, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 25-106) antibodyNUCB2 适用: 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 194-420) antibodyNUCB2 适用: 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleobindin 2 (NUCB2) (AA 341-390) antibodyNUCB2 适用: 人, 大鼠, 小鼠, 犬, 马, Cow, Pig, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1200
Application Dilutions Western blot wb: 1:200-1:400
- 说明
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Negative Control: (ABIN7236159)
Blocking Peptide: (ABIN7236159)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- NUCB2 (Nucleobindin 2 (NUCB2))
- 别名
- NUCB2 (NUCB2 产品)
- 背景
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NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109,Nucleobindin contains multiple functional domains including a signal peptide on the N-terminal side, a leucine/isoleucine rich region, a DNA binding domain and a putative nuclear targeting signal. The second half of the protein contains two Ca2+-EF-hand motifs and a leucine zipper motif in the C-terminal region. To date, two nucleobindins have been identified, namely nucleobindin 1 and nucleobindin 2.Nucleobindin 2 (NUCB2) can be found on the plasma membrane and in the cytoplasm. Putative post-translational processing of NUCB2 by the enzyme pro-hormone convertase (PC)-1/3 results in nesfatin-1, nesfatin-2 and nesfatin-3. So far, a biological activity has only been demonstrated for nesfatin-1 and its fragment, nesfatin-124-531.The gene Nesfatin/NUCB2 encoding the protein is expressed in the appetite control hypothalamic nuclei such as the paraventricular nucleus (PVN), the supraoptic nucleus, the lateral hypothalamic area and the zona increta in rats. Nefstain-1 immunoreactivity is also detected in the brain stem nuclei such as the nucleus of the solitary tract and the dorsal nucleus of the vagus.Nesfatin-1 has a substantial anorexigenic effect through the selective reduction of nesfatin/NUCB mRNA expression in the PVN of rats under starved conditions. Refeeding of rats was found to restore Nesfatin-1 reduction. A similar effect was not observed in other hypothalamic nuclei2. In humans, Nesfatin-1 levels in the saliva and serum of epilepsy patients are reduced with the use of anti-epileptic medication, but are still higher than the amounts in normal subjects. In addition, nesfatin-1 levels are decreased in human subjects with type-2 diabetes and it has been hypothesized that the observed decrement might be associated with diabetic hyperphagia3.
Alternative names: Nucleobindin-2/Nesfatin-1, NUCB2, NEFA, Prepronesfatin, DNA-binding protein NEFA, Gastric cancer antigen Zg4, HEL-S-109 - 基因ID
- 59295
- NCBI登录号
- NM_005013
- UniProt
- Q9JI85
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