NIPAL4 抗体 (Extracellular)
Quick Overview for NIPAL4 抗体 (Extracellular) (ABIN7043394)
抗原
See all NIPAL4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 242-256, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4
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特异性
- 2nd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- rat - 12,15 amino acid residues identical,Mouse - 13
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产品特性
- Anti-NIPAL4 (extracellular) Antibody is directed against an epitope of human magnesium transporter NIPA4. Anti-NIPAL4 (extracellular) Antibody (ABIN7043394, ABIN7044847 and ABIN7044848) can be used in western blot and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize NIPAL4 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EKVTTIMEMASKMKD, corresponding to amino acid residues 242-256 of human magnesium transporter NIPA4
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亚型
- IgG
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anti-NIPA-Like Domain Containing 4 (NIPAL4) (AA 9-37), (N-Term) antibody
NIPAL4 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB27750 unconjugated
anti-NIPA-Like Domain Containing 4 (NIPAL4) (AA 21-70) antibodyNIPAL4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-NIPA-Like Domain Containing 4 (NIPAL4) (AA 16-45), (N-Term) antibodyNIPAL4 适用: 人, 小鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7236151)
Blocking Peptide: (ABIN7236151)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- NIPAL4 (NIPA-Like Domain Containing 4 (NIPAL4))
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别名
- NIPAL4
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背景
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Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4,The NIPA family is currently comprised of 4 members- NIPA1-4. These members have a molecular similarity of 40 % . NIPA proteins are integral membrane proteins which function as Mg2+ transporters.The NIPA4 gene in mice is located on chromosome 11B1.1 and the human corresponding gene, hIchthyin is located on chromosome 5q33.3. Both genes share an 85 % amino acid similarity. Interestingly, NIPA2-4 show greater common identity in their ancestral invertebrate polypeptides than they do to NIPA1 suggesting different physiological roles. NIPA4 mediates Mg2+ uptake with a Km of 0.36 mM but unlike NIPA2, which is selective for Mg2+, it has been found to transport other cations as well.Mutations in the hIchthyin gene provide the basis of one form of autosomal-recessive congenital ichthyosis (ARCI). ARCI is a heterogenous group of disorders with abnormal differentiation and desquamation of the epidermis, leading to widespread scaling and erythema of the skin. To this day six homozygous mutations have been found, including one nonsense and five missense mutations, in a large number of consanguineous families presenting with ichthyosis. The function of ichthyin in skin differentiation is not fully understood but it has been suggested that because lipid dysfunctions are involved with the other ARCI disorders ichthyin might perform as a membrane receptor for trioxilins of the hepoxilin pathway.Although some NIPA proteins have been implicated as candidates for prader-willi syndrome their involvement remains unclear. Currently there is no evidence that NIPA4 is directly involved in the many complexities of the syndrome1.
Alternative names: NIPAL4, Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4 -
基因ID
- 348938
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NCBI登录号
- NM_001099287
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UniProt
- Q0D2K0
抗原
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