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CD56 抗体 (Extracellular, N-Term)

This 兔多克隆 antibody specifically detects CD56 in FACS, WB, IHC, IF, IC 和 LCI. It exhibits reactivity toward 大鼠.

Cell surface detection of NCAM1 by indirect flow cytometry in live intact human natural killer cells cells: + goat-anti-rabbit-FITC. (CD56 抗体 (Extracellular, N-Term))

FACS

Expression of NCAM1 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb frozen section using Anti-CD56/NCAM1 (extracellular) Antibody (ABIN7043350, ABIN7044686 and ABIN7044687), (1:300). (CD56 抗体 (Extracellular, N-Term))

IHC

Expression of NCAM1 in rat PC12 cells - Cell surface detection of NCAM1 in intact living rat pheochromocytoma (PC12) cells using Anti-CD56/NCAM1 (extracellular) Antibody (ABIN7043350, ABIN7044686 and ABIN7044687), (1:100). (CD56 抗体 (Extracellular, N-Term))

ICC

Western blot analysis of mouse brain (lanes 1 and 6), rat cerebellum (lanes 2 and 7), rat eye (lanes 3 and 8), human SH-SY5Y brain neuroblastoma (lanes 4 and 9) and PC12 rat pheochromocytoma (lanes 5 and 10) cells lysates: - 1-5. (CD56 抗体 (Extracellular, N-Term))

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产品编号 ABIN7043350

发货至: 中国

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Quick Overview for CD56 抗体 (Extracellular, N-Term) (ABIN7043350)

抗原

See all CD56 (NCAM1) 抗体

CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

适用

大鼠

宿主

兔

克隆类型

多克隆

标记

This CD56 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

质量等级

KO Validated

Discover our top product NCAM1 Primary Antibody

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AA 261-276, Extracellular, N-Term

原理

A Rabbit Polyclonal Antibody to Neural Cell Adhesion Molecule 1 (CD56)

交叉反应

人, 小鼠, 大鼠

预测反应

Mouse - identical, human - 13,15 amino acid residues identical

产品特性

Anti-CD56/NCAM1 (extracellular) Antibody (ABIN7043350, ABIN7044686 and ABIN7044687) is a highly specific antibody directed against rat Neural cell adhesion molecule 1. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NCAM1 from rat, mouse and human samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

(C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1

亚型

IgG
anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 759-858) antibody
NCAM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibody
NCAM1 适用: 人, 小鼠, 猴 FACS, WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5B1G11 unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (Internal Region) antibody
Verified NCAM1 适用: 人 FACS, WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibody
NCAM1 适用: 人, 大鼠 FACS, WB, IHC, IF 宿主: 小鼠 Monoclonal NCAM1-795 unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 568-708) antibody
NCAM1 适用: 人, 小鼠 WB, ELISA 宿主: 小鼠 Monoclonal 1F6F10 unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) antibody (PerCP)
NCAM1 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-188 PerCP

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 701-800) antibody
NCAM1 适用: 人, 大鼠, 小鸡 FACS, WB, ELISA, IHC (p), IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 829-863), (C-Term) antibody
NCAM1 适用: 人 FACS, WB 宿主: 兔 Polyclonal RB51136 unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 796-830), (C-Term) antibody
NCAM1 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB51135 unconjugated

anti-Neural Cell Adhesion Molecule 1 (NCAM1) (AA 340-608) antibody
NCAM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:200-1:400
FC: 5 μg per reaction
ICC: 1:100
IHC: 1:300
IP: The optimal concentration should be determined by the user

说明

Negative Control: (ABIN7236018)
Blocking Peptide: (ABIN7236018)

限制

仅限研究用
状态

Lyophilized

溶解方式

25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

浓度

0.8 mg/mL

缓冲液

PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
抗原

CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

别名

NCAM1

背景

Synonyms: Neural cell adhesion molecule 1, NCAM-1
Description: Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3. NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4.Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

基因ID

24586

NCBI登录号

NM_000615

UniProt

P13596