Mucolipin 3 抗体 (Intracellular) (Atto 594)
Quick Overview for Mucolipin 3 抗体 (Intracellular) (Atto 594) (ABIN7043339)
抗原
See all Mucolipin 3 (Mcoln3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 528-542, Intracellular
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原理
- A Rabbit Polyclonal Antibody to TRPML3 Channel Conjugated to the Fluorescent Dye ATTO-594
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特异性
- Intracellular, C-terminus (cytoplasmic)
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - identical
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产品特性
- Anti-TRPML3 (Mucolipin 3) Antibody (ABIN7043338 and ABIN7044043) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TRPML3 from human, rat and mouse samples. \nAnti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody (ABIN7043339) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-TRPML3 (Mucolipin 3)-ATTO Fluor-594 Antibody has been tested in immunohistochemical applications and is specially suited to experiments requiring simultaneous labeling of different markers.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CKDLPNSGKYRLEDD, corresponding to amino acid residues 528-542 of mouse TRPML3
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亚型
- IgG
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anti-Mucolipin 3 (Mcoln3) (AA 201-250) antibody
Mcoln3 适用: 人, 大鼠, 豚鼠, 兔, 犬, 马, Hamster, 猴, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Mucolipin 3 (Mcoln3) (AA 215-264) antibodyMcoln3 适用: 人, 小鼠, 大鼠, 豚鼠, 兔, Cow, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Mucolipin 3 (Mcoln3) (AA 528-542), (Intracellular) antibodyMcoln3 适用: 小鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7235979)
Blocking Peptide: (ABIN7235979)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Mucolipin 3 (Mcoln3)
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别名
- MCOLN3
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背景
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Mcoln3,The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH 1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.
Alternative names: TRPML3 (Mucolipin 3), Mcoln3 -
基因ID
- 171166
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NCBI登录号
- NM_018298
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UniProt
- Q8R4F0
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途径
- Sensory Perception of Sound
抗原
-
