MCU 抗体 (Mitochondrial Matrix, N-Term)
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Quick Overview for MCU 抗体 (Mitochondrial Matrix, N-Term) (ABIN7043332)
抗原
See all MCU 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 191-206, Mitochondrial Matrix, N-Term
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原理
- A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,mouse - 15,16 amino acid residues identical
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产品特性
- Anti-MCU Antibody (ABIN7043332, ABIN7044117 and ABIN7044118) is a highly selective antibody directed against an epitope of the human mitochondrial calcium uniporter. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter
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亚型
- IgG
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anti-Mitochondrial Calcium Uniporter (MCU) (AA 300-350) antibody
MCU 适用: 人, 小鼠 IHC, WB, ICC, IP 宿主: 兔 Monoclonal BLR355N unconjugated Recombinant Antibody
anti-Mitochondrial Calcium Uniporter (MCU) (AA 1-351) antibodyMCU 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uniporter (MCU) (AA 155-233) antibodyMCU 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uniporter (MCU) (Internal Region) antibodyMCU 适用: 人, 小鼠 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uniporter (MCU) (AA 51-351) antibodyMCU 适用: 人, 小鼠, 大鼠, 猴 ELISA, IHC, WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:300
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235956)
Blocking Peptide: (ABIN7235956)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- MCU (Mitochondrial Calcium Uniporter (MCU))
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别名
- MCU
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背景
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Synonyms: Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42
Description: Mitochondrial calcium uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca2+) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits1-3.Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca2+ oscillation, resulting in an increase of muscle contraction2.Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix1,3.
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基因ID
- 90550
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NCBI登录号
- NM_138357
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UniProt
- Q8NE86
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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