MCU 抗体 (Mitochondrial Matrix)
Quick Overview for MCU 抗体 (Mitochondrial Matrix) (ABIN7043332)
抗原
See all MCU 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 191-206, Mitochondrial Matrix
-
原理
- A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uniporter
-
特异性
- N-terminus, mitochondrial matrix
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Rat,mouse - 15,16 amino acid residues identical
-
产品特性
- Anti-MCU Antibody (ABIN7043332, ABIN7044117 and ABIN7044118) is a highly selective antibody directed against an epitope of the human mitochondrial calcium uniporter. The antibody can be used in western blot analysis. It has been designed to recognize MCU from human, mouse, and rat samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: CIEQHQLNKERELIER, corresponding to amino acid residues 191-206 of human mitochondrial calcium uniporter
-
亚型
- IgG
-
-
anti-Mitochondrial Calcium Uniporter (MCU) (AA 1-351) antibody
MCU 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uniporter (MCU) (AA 155-233) antibodyMCU 适用: 人 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Mitochondrial Calcium Uniporter (MCU) (Internal Region) antibodyMCU 适用: 人, 小鼠 ELISA, IHC, WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
-
说明
-
Negative Control: (ABIN7235956)
Blocking Peptide: (ABIN7235956)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- MCU (Mitochondrial Calcium Uniporter (MCU))
-
别名
- MCU
-
背景
-
Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42,Mitochondrial calcium uniporter (MCU) is a 480 kDa mitochondrial inner membrane protein, a multimer that forms a calcium (Ca2+) channel in the lipid bilayer. MCU is one of the most important and highly selective channels responsible for mitochondrial calcium uptake. This uptake plays an important role in cell energy balance and in cell fate determination.The MCU protein is comprised of Mcu which functions as the pore-forming and calcium-conducting subunit, encoded by the Ccdc109a gene. The other units- MICU1 (calcium uptake protein 1, mitochondrial), MICU2 (calcium uptake protein 2, mitochondrial), EMRE (essential MCU regulator, mitochondrial), MCUR1 (mitochondrial calcium uniporter regulator 1) and MCUb (calcium uniporter regulatory subunit MCUb, mitochondrial) all function as regulatory subunits1-3.Studies have demonstrated that knockdown of Mcu uniport in isolated cardiomyocytes enhances peak levels in cytosolic Ca2+ oscillation, resulting in an increase of muscle contraction2.Mcu pore-forming subunit is a homo-oligomer with two coil-coiled domains and two transmembrane domains (TM) separated by a short hydrophilic loop enriched in acidic amino acids. The second transmembrane helices from five Mcu subunits form a hydrophilic pore in the inner membrane of the mitochondria. The DXXE motif forms the pore entrance in the inter-membrane space. The N- and the C-terminus of MCU face the matrix1,3.
Alternative names: MCU, Mitochondrial calcium uniporter, HsMCU, Coiled-coil domain-containing protein 109A, Ccdc109a, C10orf42 -
基因ID
- 90550
-
NCBI登录号
- NM_138357
-
UniProt
- Q8NE86
-
途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
-
