MCHR1 抗体 (Extracellular)
Quick Overview for MCHR1 抗体 (Extracellular) (ABIN7043320)
抗原
See all MCHR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 7-20, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- human - 13,Mouse - 16,17 amino acid residues identical
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产品特性
- Anti-MCHR1 (extracellular) Antibody (ABIN7043320 and ABIN7044603) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1
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亚型
- IgG
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anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) antibody
MCHR1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (AA 14-113) antibodyMCHR1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D7 unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (AA 396-422), (C-Term) antibodyMCHR1 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB28532 unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (N-Term) antibodyMCHR1 适用: 人, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (N-Term) antibodyMCHR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (AA 1-422) antibodyMCHR1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (AA 399-426), (C-Term) antibodyMCHR1 适用: 人 WB, IHC (p), FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (AA 75-180) antibodyMCHR1 适用: 大鼠 ELISA, IHC (p), IF (p), IF (cc), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Melanin-Concentrating Hormone Receptor 1 (MCHR1) (Internal Region) antibodyMCHR1 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IHC
Negative Control: (ABIN7235912)
Blocking Peptide: (ABIN7235912)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- MCHR1 (Melanin-Concentrating Hormone Receptor 1 (MCHR1))
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别名
- MCHR1
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背景
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Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1,Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.
Alternative names: MCHR1, Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1 -
基因ID
- 83567
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NCBI登录号
- NM_005297
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UniProt
- P97639
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途径
- Feeding Behaviour
抗原
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