LPAR5 抗体 (Extracellular)
Quick Overview for LPAR5 抗体 (Extracellular) (ABIN7043316)
抗原
See all LPAR5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 261-276, Extracellular
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原理
- A Rabbit Polyclonal Antibody to LPA Receptor 5
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特异性
- 3rd extracellular loop
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交叉反应 （详细）
- Will not recognize rat or mouse LPA5 receptor.
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预测反应
- Mouse,Rat - not recommended for use with mouse,rat samples
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产品特性
- Anti-Human LPAR5 (extracellular) Antibody is directed against an epitope of the human LPA5 receptor. Anti-Human LPAR5 (extracellular) Antibody (ABIN7043316, ABIN7044574 and ABIN7044575) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA5 receptor from human samples only.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RSKLVAASVPARDRVR, corresponding to amino acid residues 261-276 of human LPA5 receptor
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亚型
- IgG
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anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (AA 310-326) antibody
LPAR5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Extracellular Domain) antibodyLPAR5 适用: 人, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Extracellular Domain) antibodyLPAR5 适用: 人 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Cytoplasmic Domain) antibodyLPAR5 适用: 人, 小鼠 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 5 (LPAR5) (Internal Region) antibodyLPAR5 适用: 人 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- LPAR5 (Lysophosphatidic Acid Receptor 5 (LPAR5))
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别名
- LPAR5
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背景
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Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93,LPA (lysophosphatidic acid) mediates cellular differentiation, growth, survival and motility through two subgroups of G-protein coupled receptors (GPCRs): The Edg (endothelial differentiation gene) family - LPA1, LPA2, LPA3 and the non-Edg family - LPA4, LPA5, LPA61.Like all GPCRs, LPA receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail1.Alkyl-LPA, commonly found in platelets activates LPA5, thus explaining the high expression of the receptor in platelets2. LPA is expressed in astrocytes, and sensory and motor neurons in the spinal cord. It is also detected in spleen, heart, placenta, lliver and colon3.LPA5 activation leads to cAMP production, however, it does so in a Gs independent manner4,5. The receptor couples Gq, thus leading to Ca2+ mobilization4.Activation of LPA5 was also shown to initiate neuropathic pain in mouse models. LPA5 knockout mice do not develop neuropathic pain6.
Alternative names: LPAR5, Lysophosphatidic acid receptor 5, LPA-5, GPR92, GPR93 -
基因ID
- 57121
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NCBI登录号
- NM_020400
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UniProt
- Q9H1C0
抗原
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