LPAR4 抗体 (Extracellular)
Quick Overview for LPAR4 抗体 (Extracellular) (ABIN7043315)
抗原
See all LPAR4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 3-16, Extracellular
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原理
- A Rabbit Polyclonal Antibody to LPA Receptor 4 (P2Y9)
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特异性
- Extracellular, N-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - identical,human - 13,14 amino acid residues identical
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产品特性
- Anti-LPAR4 (P2Y9) (extracellular) Antibody is directed against an epitope of the mouse LPA4 receptor. Anti-LPAR4 (P2Y9) (extracellular) Antibody (ABIN7043315, ABIN7044572 and ABIN7044573) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize LPA4 receptor from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DRRFIDFQFQDLNS, corresponding to amino acid residues 3-16 of mouse LPA4 receptor
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亚型
- IgG
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anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (AA 175-270) antibody
LPAR4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (AA 1-43) antibodyLPAR4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (N-Term) antibodyLPAR4 适用: 人, 小鼠, 大鼠, Bat, 猴, Hamster IHC, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (Cytoplasmic Domain) antibodyLPAR4 适用: 人, 小鼠, 大鼠, 犬, 兔, 马, Bat, 猴, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (C-Term) antibodyLPAR4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 4 (LPAR4) (C-Term) antibodyLPAR4 适用: 人, 犬, Cow, Pig, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:500
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说明
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Negative Control: (ABIN7235905)
Blocking Peptide: (ABIN7235905)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- LPAR4 (Lysophosphatidic Acid Receptor 4 (LPAR4))
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别名
- LPAR4
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背景
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Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23,Lysophosphatidic acid (LPA) is a bioactive lipid molecule with a phosphate, a glycerol, and a fatty acid in its structure. The cellular effects of LPA include proliferation, migration, cytokine secretion, and morphological change. These pleiotropic actions allow LPA to participate in a wide variety of biological processes, such as brain development, oncogenesis, wound healing and cancer progression1. To date, at least 6 subtypes of LPA receptor have been identified. The LPA1-3 receptors are members of the endothelial cell differentiation gene (Edg) family. LPA4, a member of the purinergic receptor family, and the recently identified LPA5 are structurally distant from the canonical Edg LPA1-3 receptors. LPA4 and LPA5 are linked to Gq, G12/13 and Gs but not Gi, while LPA1-3 all couple to Gi in addition to Gq and G12/132.LPA4 (P2Y9/GPR23) was cloned as an orphan GPCR and is closely related to the purinergic P2Y receptors3. Like all GPCRs it has seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is on the cytoplasmic side4.LPA4 is weakly expressed, there are high levels only in ovary2.LPA4-deficient mice display abnormalities in the blood vascular systems, which caused lethality of embryos and neonates5.
Alternative names: LPAR4 (P2Y9), Lysophosphatidic acid receptor 4, LPA receptor 4, LPA-4, P2Y purinoceptor 9, Purinergic receptor 9, P2RY9, GPR23 -
基因ID
- 78134
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NCBI登录号
- NM_005296
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UniProt
- Q8BLG2
抗原
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