LPAR1 抗体 (Extracellular)
Quick Overview for LPAR1 抗体 (Extracellular) (ABIN7043311)
抗原
See all LPAR1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 36-50, Extracellular
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原理
- A Rabbit Polyclonal Antibody to LPA Receptor 1 (EDG2)
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 14,15 amino acid residues identical
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产品特性
- Anti-LPAR1 (EDG2) (extracellular) Antibody (ABIN7043311, ABIN7044566 and ABIN7044567) is a highly specific antibody directed against an epitope of the rat LPA1 receptor. The antibody can be used in western blot analysis. It has been designed against an extracellular domain and thus is highly suitable for detecting the receptor in living cells. It has been designed to recognize LPA1 receptor from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor
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亚型
- IgG
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anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 281-364) antibody
LPAR1 适用: 人, 小鼠 WB, ELISA, IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) antibodyLPAR1 适用: 人 WB, IF, FACS 宿主: 小鼠 Monoclonal 1G6 unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (N-Term) antibodyLPAR1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 316-364) antibodyLPAR1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 342-359) antibodyLPAR1 适用: 人, 小鼠, 大鼠, 猴, Bat, 小鸡, Cow, 犬, Hamster, 马, Pig, 绵羊, 非洲爪蟾 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (Cytoplasmic Domain) antibodyLPAR1 适用: 人, 小鼠, 大鼠, 猴, Bat, 小鸡, Cow, 犬, Hamster, 马, Pig, 绵羊, 非洲爪蟾, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (N-Term) antibodyLPAR1 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (N-Term) antibodyLPAR1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) antibodyLPAR1 适用: 人 WB, ELISA 宿主: 兔 Monoclonal 4C3 unconjugated Recombinant Antibody
anti-Lysophosphatidic Acid Receptor 1 (LPAR1) (AA 5-54) antibodyLPAR1 适用: 人, 大鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000
Application Dilutions Western blot wb: 1:400
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说明
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Negative Control: (ABIN7235898)
Blocking Peptide: (ABIN7235898)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))
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别名
- LPAR1
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背景
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Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1,Lysophosphatidic acid is a naturally occurring lysophospholipid that activates diverse cellular actions in various cells including proliferation and morphological changes. There are currently three known genes that encode proteins of the LPA receptor family- Edg2, Edg4 and Edg7.Lysophosphatidic acid receptor 1 (also known as Lpa1), encoded by Edg2, belongs to G-coupled protein receptor (GPCR) superfamily. All three proteins share 50-54 % identical amino-acids. Lpa1 mostly activates the Gi pathway that leads to inhibition of adenylate cyclase and thus inhibition of cAMP production. Through this pathway, Lpa1 also initiates the Ras/MAP kinase cascade which is primarily responsible for increased cell proliferation1. Interestingly, binding of lysophosphatidic acid to the G13 G-protein activates the small GTP-binding protein Rho and induces the rapid remodeling of the actin cytoskeleton2.Lpa1 has been implicated in the pathogenesis of metastatic bone cancer. Silencing of Lpa1 expression in breast and ovarian cancer cells significantly alters the progression of bone metastasis through reduction of tumor burden and inhibition of bone resorption. A specific Lpa1 antagonist exhibits specificity for tumor cell-platelet interaction without abrogating normal platelet function3.
Alternative names: LPAR1 (EDG2), Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1 -
基因ID
- 116744
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NCBI登录号
- NM_057159
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途径
- Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration
抗原
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