LRRC4B 抗体 (Extracellular, N-Term)
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Quick Overview for LRRC4B 抗体 (Extracellular, N-Term) (ABIN7886681)
抗原
See all LRRC4B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 498-511, Extracellular, N-Term
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原理
- A Rabbit Polyclonal Antibody to NGL-3/LRRC4B
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - identical, human - 13 out of 14 amino acid residues identical
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产品特性
- Anti-/LRRC4B (extracellular) Antibody (ABIN7043300, ABIN7044736 and ABIN7044737) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3
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亚型
- IgG
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anti-Leucine Rich Repeat Containing 4B (LRRC4B) (AA 81-110), (N-Term) antibody
LRRC4B 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB27571 unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: 1:200
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235861)
Blocking Peptide: (ABIN7235861)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))
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别名
- LRRC4B
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背景
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Synonyms: Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4
Description: NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.Mice lacking NGL-3 (Ngl3-/-) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.
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基因ID
- 272381
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NCBI登录号
- NM_001080457
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UniProt
- P0C192
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途径
- Synaptic Membrane
抗原
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