LRRC4B 抗体 (Extracellular)
LRRC4B
适用: 小鼠
WB, IHC, IF
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all LRRC4B 抗体
- LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))
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抗原表位
- AA 498-511, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This LRRC4B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to NGL-3/LRRC4B
- 特异性
- Extracellular, N-terminus
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- Rat - identical, human - 13 out of 14 amino acid residues identical
- 产品特性
- Anti-NGL-3/LRRC4B (extracellular) Antibody (ABIN7043300, ABIN7044736 and ABIN7044737)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3
- 亚型
- IgG
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: BLP-NR163
Blocking Peptide: BLP-NR163
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))
- 别名
- LRRC4B (LRRC4B 产品)
- 背景
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Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4,NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.Mice lacking NGL-3 (Ngl3-/-) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.
Alternative names: NGL-3/LRRC4B, Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4 - 基因ID
- 272381
- NCBI登录号
- NM_001080457
- UniProt
- P0C192
- 途径
- Synaptic Membrane
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