LRRC26 抗体 (Intracellular)
Quick Overview for LRRC26 抗体 (Intracellular) (ABIN7043299)
抗原
See all LRRC26 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 301-314, Intracellular
-
原理
- A Rabbit Polyclonal Antibody to LRRC26
-
特异性
- Intracellular, C-terminus
-
交叉反应
- 小鼠, 大鼠
-
交叉反应 （详细）
- Won't recognize human LRRC26.
-
预测反应
- Mouse - 12,14 amino acid residues identical
-
产品特性
- Anti-LRRC26 Antibody (ABIN7043299, ABIN7044982 and ABIN7044983) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LRRC26 from rat and mouse samples. It does not recognize the protein from human samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: (C)RRTTVRHLIRRQPD, corresponding to amino acid residues 301-314 of rat LRRC26
-
亚型
- IgG
-
-
anti-Leucine Rich Repeat Containing 26 (LRRC26) (AA 209-258) antibody
LRRC26 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Leucine Rich Repeat Containing 26 (LRRC26) (Middle Region) antibodyLRRC26 适用: 人, 犬 WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200-1:400
-
说明
-
Negative Control: (ABIN7235852)
Blocking Peptide: (ABIN7235852)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 0.2 mL double distilled water (DDW).
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- LRRC26 (Leucine Rich Repeat Containing 26 (LRRC26))
-
别名
- LRRC26
-
背景
-
Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC,BK (KCa1.1) channels are critically involved in diverse physiological processes in both electrically excitable and non-excitable cells.Leucine-rich repeat containing protein 26 (also known as BK channel auxiliary gamma subunit or LRRC26) is a single spanning transmembrane protein of ~37 kDa. LRRC26 protein contains five major leucine rich repeats in its extracellular domain which are all required for modulating BK channel activity. The single transmembrane segment of LRRC26 is required for co-assembly with the BKα subunit complex.When LRRC26 is acutely knocked down in LNCaP cells, the voltage-dependent activation of endogenous BK current shifts from a low to a high V1/2 value, whereas recombinant expression of LRRC26 cDNA in naive cells (such as PC3 prostate cancer cells and HEK 293 cells) normally lacking LRRC26 shifts BK channel activation from high to low V1/2 values, reproducing the channel behavior observed in LNCaP cells. This findings indicate that LRRC26 is sufficient to impart low voltage gating behavior to wild-type pore-forming, BKα subunits.LRRC26 lowers the energy barrier for activation of BK channels, and is able to stabilize channel opening in response to voltage, thereby maintaining channels in the open state to a much greater extent at any given membrane potential1.LRRC26 is expressed in cerebral artery myocytes where it is primarily plasma membrane localized and associated with BKα subunits. LRRC26 serves as an arterial myocyte BK channel auxiliary γ subunit that elevates voltage and apparent Ca2+ sensitivity to induce vasodilation2.
Alternative names: LRRC26, Leucine-rich repeat-containing protein 26, BK channel auxiliary gamma subunit LRRC26, Cytokeratin-associated protein in cancer, CAPC -
基因ID
- 311803
-
NCBI登录号
- NM_001013653
-
UniProt
- Q6P7C4
抗原
-
