我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

HRH4 抗体 (Extracellular)

Name: HRH4 抗体 (Extracellular)
SKU: ABIN7043272
Availability: OutOfStock

This anti-HRH4 antibody is a 兔多克隆 antibody detecting HRH4 in WB, FACS 和 LCI. Suitable for 人.

Western blot analysis of human chronic myelogenous leukemia (K562) (lanes 1 and 3) and human promyelocytic leukemia (HL-60) (lanes 2 and 4) cell lysates: - 1,2. (HRH4 抗体 (Extracellular))

Cell surface detection of Histamine H4 receptor in live intact Jurkat (acute T-cell leukemia) cell lines: (black line) Unstained cells + goat-anti-rabbit-PE. (HRH4 抗体 (Extracellular))

FACS

2 images

产品编号 ABIN7043272

发货至: 中国

Quick Overview for HRH4 抗体 (Extracellular) (ABIN7043272)

抗原

See all HRH4 抗体

HRH4 (Histamine Receptor H4 (HRH4))

适用

人

宿主

兔

克隆类型

多克隆

标记

This HRH4 antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Discover our top product HRH4 Primary Antibody

抗原表位
15

8

7

7

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 75-87, Extracellular

原理

A Rabbit Polyclonal Antibody to Human Histamine H4 Receptor

特异性

1st extracellular loop

预测反应

Won't recognize rat,mouse H4 Histamine Receptor

产品特性

Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody is directed against an epitope located at the 1st extracellular loop of the human histamine H4 receptor. Anti-Human Histamine H4 Receptor (HRH4) (extracellular) Antibody (ABIN7043272, ABIN7044510 and ABIN7044511) can be used in western blot and indirect flow cytometry applications, and recognizes H4R from human samples.

纯化方法

Affinity purified on immobilized antigen.

免疫原

Immunogen: Synthetic peptide
Immunogen Sequence: HTLFEWDFGKEIC, corresponding to amino acids 75-87 of human HRH4

亚型

IgG
anti-Histamine Receptor H4 (HRH4) (C-Term) antibody
HRH4 适用: 人 ELISA, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 204-292) antibody
HRH4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (N-Term) antibody
KO Validated HRH4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (Cytoplasmic Domain) antibody
HRH4 适用: 人, 猴 IHC, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 285-390) antibody
HRH4 适用: 人 WB, ELISA, ICC, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 101-200) antibody
HRH4 适用: 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) antibody
HRH4 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 359-390), (C-Term) antibody
HRH4 适用: 人 WB 宿主: 兔 Polyclonal RB1073 unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 142-191) antibody
HRH4 适用: 人, Pig WB 宿主: 兔 Polyclonal unconjugated

anti-Histamine Receptor H4 (HRH4) (AA 194-304) antibody
HRH4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:400

限制

仅限研究用
状态

Lyophilized

溶解方式

0.2 mL double distilled water (DDW).

浓度

1 mg/mL

缓冲液

PBS pH 7.4

储存条件

4 °C,-20 °C

储存方法

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
抗原

HRH4 (Histamine Receptor H4 (HRH4))

别名

HRH4

背景

H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1- H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Histamine H4 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, mobilisation of calcium from intracellular stores and activation of the mitogen-activated protein kinase (MAPK) cascade.1,2Histamine H4 receptor is largely expressed in haemopoietic cells including mast cells, eosinophils, dendritic cells and T lymphocytes. H4 receptors modulate eosinophil migration and the selective recruitment of mast cells leading to amplification of histamine-mediated immune responses. In addition, H4 receptors are involved in dendritic cell activation and the regulation of T lymphocyte cytokine production.2,3These studies indicate that histamine H4 receptor is an attractive therapeutic target for the treatment of inflammatory disorders, such as allergy, asthma, chronic pruritus and autoimmune diseases.2,3

Alternative names: Histamine H4 Receptor (HRH4), H4R, HH4R, G-protein coupled receptor 105, GPCR105, GPRv53, AXOR35

基因ID

59340

NCBI登录号

NM_001143828

UniProt

Q9H3N8