HRH3 抗体 (AA 228-242)
Quick Overview for HRH3 抗体 (AA 228-242) (ABIN7043271)
抗原
See all HRH3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 228-242
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原理
- A Rabbit Polyclonal Antibody to Histamine H3 Receptor
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特异性
- 3rd intracellular loop
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human 14,16 amino acid residues identical
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产品特性
- Anti-Histamine H3 Receptor (HRH3) Antibody is directed against an epitope of the rat histamine H3 receptor. Anti-Histamine H3 Receptor (HRH3) Antibody (ABIN7043271 and ABIN7044509) can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize H3R from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RTRLRLDGGREAGPE, corresponding to amino acids 228-242 of rat HRH3
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亚型
- IgG
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anti-Histamine Receptor H3 (HRH3) (Cytoplasmic Domain) antibody
HRH3 适用: 人, 猴 IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (AA 236-332) antibodyHRH3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (C-Term) antibodyHRH3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (AA 250-350) antibodyHRH3 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) antibodyHRH3 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (AA 121-220) antibodyHRH3 适用: 人, 大鼠 WB, IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (Cytoplasmic Domain) antibodyHRH3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (Cytoplasmic Domain) antibodyHRH3 适用: 人, 大鼠, 小鼠, Cow, 马, 豚鼠, Hamster, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Histamine Receptor H3 (HRH3) (AA 257-359) antibodyHRH3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D7 unconjugated
anti-Histamine Receptor H3 (HRH3) (C-Term) antibodyHRH3 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235758)
Blocking Peptide: (ABIN7235758)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- HRH3 (Histamine Receptor H3 (HRH3))
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别名
- HRH3
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背景
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H3R, HH3R, GPCR97,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H3 receptor couples to Gi/G0 proteins and receptor activation leads to inhibition of adenylate cyclase, activation of the mitogen-activated protein kinase (MAPK) cascade and inhibition of the Na+/H+ exchanger.1,2H3 receptors are expressed primarily in the central nervous system (CNS) where they are located in presynaptic membranes of histaminergic neurons, where they negatively regulate the synthesis and release of histamine. In addition, H3 receptors are also located on nonhistaminergic neurons, where they regulate the release of other amines such as dopamine, serotonin, and norepinephrine.2,3Based on these studies, a central role for H3 receptors has been proposed in disorders involving cognition such as attention deficit and hyperactivity disorder (ADHD), Alzheimer disease and Schizophrenia, as well as sleep and energy homeostasis (i.e. obesity) disorders.2,3
Alternative names: Histamine H3 Receptor (HRH3), H3R, HH3R, GPCR97 -
基因ID
- 85268
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NCBI登录号
- NM_007232
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UniProt
- Q9QYN8
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途径
- cAMP Metabolic Process, Feeding Behaviour
抗原
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