ChT 抗体 (Extracellular)
Quick Overview for ChT 抗体 (Extracellular) (ABIN7043267)
抗原
See all ChT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 299-311, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Choline Transporter (SLC5A7)
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特异性
- 4th extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat,human - 12,13 amino acid residues identical
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产品特性
- Anti-Choline Transporter (SLC5A7) (extracellular) Antibody is directed against an epitope of mouse CHT. Anti-Choline Transporter (SLC5A7) (extracellular) Antibody (ABIN7043267 and ABIN7044218) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CHT from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DWNQTAYGYPDPK, corresponding to amino acid residues 299-311 of mouse Choline transporter
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亚型
- IgG
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anti-High Affinity Choline Transporter (ChT) (AA 421-580) antibody
ChT 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-High Affinity Choline Transporter (ChT) (AA 501-580) antibodyChT 适用: 小鼠, 人, 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal 62-2E8 unconjugated
anti-High Affinity Choline Transporter (ChT) (AA 468-517) antibodyChT 适用: 小鼠, 人, 大鼠, 小鸡, Cow, 豚鼠, 兔, 马, Hamster, Pig, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-High Affinity Choline Transporter (ChT) antibody (PE)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 PE
anti-High Affinity Choline Transporter (ChT) antibodyChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 unconjugated
anti-High Affinity Choline Transporter (ChT) antibody (Biotin)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 Biotin
anti-High Affinity Choline Transporter (ChT) antibody (FITC)ChT 适用: 小鸡 FACS 宿主: 小鼠 Monoclonal CT1 FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- ChT (High Affinity Choline Transporter (ChT))
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别名
- SLC5A7
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背景
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Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1,In cholinergic neurons, acetylcholine is synthesized from choline and acetyl-coenzyme A (acetyl-CoA). Following depolarization, acetylcholine is released to the synaptic cleft via synaptic vesicles and activates muscarinic and nicotinic receptors located on postsynaptic membranes1,2. Acetylcholine is then rapidly hydrolyzed into choline and acetate. Since choline is not synthesized de novo and thereby only made available through diet, choline is recycled back into the cells in order to regenerate acetylcholine. For this purpose, choline is taken up by the high affinity choline transporter (CHT)1,2.CHT has thirteen transmembrane domains, an extracellular N-terminus and an intracellular C-terminus3,4. CHT belongs to the Na+-dependent glucose transporter family (SLC5). The activity of the transporter can be confirmed by its sensitivity to hemicholimium-3 (HC-3) which inhibits the transport of choline with a Ki of 10-100 nM1. The activity of CHT reflects that of neurons1,5 i.e. increased CHT activity indicates an increase in neuronal activity. The increase in the activity of the transporter is due to the increase in the number of transporters and not due to the increase in activity per se. In addition CHT activity is also regulated by second messengers. Phosphorylation of the transporter also seems to determine the activity as it has many consensus phosphorylation sites present in the C-terminal1,2.Expression studies reveal that CHT is expressed solely in cholinergic neurons making the transporter a useful marker for detecting these types of neurons6.
Alternative names: Choline Transporter (SLC5A7), Na+/Cl--Dependent high affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, CHT1 -
基因ID
- 63993
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NCBI登录号
- NM_001305005
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UniProt
- Q8BGY9
抗原
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