GLRB 抗体 (AA 394-405)
Quick Overview for GLRB 抗体 (AA 394-405) (ABIN7043260)
抗原
See all GLRB 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 394-405
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原理
- A Rabbit Polyclonal Antibody to Glycine Receptor β
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特异性
- 2nd intracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-Glycine Receptor beta Antibody is directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (ABIN7043260, ABIN7044402 and ABIN7044403) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRbeta
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亚型
- IgG
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anti-Glycine Receptor, beta (GLRB) (AA 23-160) antibody
GLRB 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3B8A8 unconjugated
anti-Glycine Receptor, beta (GLRB) (AA 51-150) antibodyGLRB 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glycine Receptor, beta (GLRB) (AA 355-472) antibodyGLRB 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Glycine Receptor, beta (GLRB) (AA 102-132), (N-Term) antibodyGLRB 适用: 人, 小鼠 WB, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235724)
Blocking Peptide: (ABIN7235724)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GLRB (Glycine Receptor, beta (GLRB))
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别名
- GLRB
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背景
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Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ,The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.
Alternative names: Glycine Receptor beta , Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRbeta -
基因ID
- 25456
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NCBI登录号
- NM_000824
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UniProt
- P20781
抗原
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