GRIP1 抗体 (Intracellular)
Quick Overview for GRIP1 抗体 (Intracellular) (ABIN7043227)
抗原
See all GRIP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 963-977, Intracellular
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原理
- A Rabbit Polyclonal Antibody to GRIP1
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特异性
- Intracellular, C-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-GRIP1 Antibody (ABIN7043227, ABIN7045158 and ABIN7045159) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize GRIP1 from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RKSVTLRKMKQEIKE, corresponding to amino acid residues 963-977 of rat GRIP1
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亚型
- IgG
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235622)
Blocking Peptide: (ABIN7235622)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GRIP1 (Glutamate Receptor Interacting Protein 1 (GRIP1))
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别名
- GRIP1
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背景
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Glutamate receptor interacting protein 1,GRIP1 (glutamate receptor interacting protein 1) is a neuron-enriched scaffolding protein that plays an important role in regulating synaptic trafficking, synaptic strength and synaptic organization and transmission of AMPA receptor 2 and 3 (GluA2/3) 1,2. GRIP1 structure contains 7 PDZ domains responsible for protein-protein interactions. GRIP1 forms homodimers that can bind and simultaneously organize and assemble protein complexes via PDZ domains1,2.GRIP1 is abundantly expressed in glutamatergic and GABAergic synapses. In addition, GRIP1 expression can be found in muscle cells and in the epidermis1,3. Studies on GRIP1 knockout mice have revealed multi-organ developmental abnormalities. These mice die due to hemorrhage and hypovolemia in the embryonic or early post-natal period2.Mutations in GRIP1 protein resulting in a gain-of-function phenotype are linked to autism, while neuronal-specific loss-of-function mice have prosocial behavior. Other point mutations have been associated with schizophrenia and congenital kidney disease2,3.
Alternative names: GRIP1, Glutamate receptor interacting protein 1 -
基因ID
- 84016
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NCBI登录号
- NM_001178074
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UniProt
- P97879
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway
抗原
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