Glucagon Receptor 抗体 (Extracellular)
Quick Overview for Glucagon Receptor 抗体 (Extracellular) (ABIN7043218)
抗原
See all Glucagon Receptor (GCGR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 200-212, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Glucagon Receptor
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特异性
- 1st extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 12,13 amino acid residues identical
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产品特性
- Anti-Glucagon Receptor (extracellular) Antibody is directed against an epitope of the rat glucagon receptor. Anti-Glucagon Receptor (extracellular) Antibody (ABIN7043218, ABIN7044410 and ABIN7044411) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GCGR from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KTRYSQKIGDDLS, corresponding to amino acid residues 200-212 of rat GCGR
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亚型
- IgG
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anti-Glucagon Receptor (GCGR) (AA 11-110) antibody
GCGR 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (AA 26-136) antibodyGCGR 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (AA 1-140) antibodyGCGR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (N-Term) antibodyGCGR 适用: 人, Cow, 猴, 犬, Hamster, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (Internal Region) antibodyGCGR 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (Extracellular Domain) antibodyGCGR 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) (N-Term) antibodyGCGR 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glucagon Receptor (GCGR) antibodyGCGR 适用: 人 IF, BR 宿主: 小鼠 Monoclonal hGR-2 F6 unconjugated Recombinant Antibody
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235603)
Blocking Peptide: (ABIN7235603)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Glucagon Receptor (GCGR)
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别名
- GCGR
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背景
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GCGR, GLR,The glucagon receptor (GCGR) is one of the 15 members of the secretin-like (class B) family of G-protein-coupled receptors (GPCRs) in humans. Glucagon is a highly conserved 29 amino acid protein processed from proglucagon by PC2 in pancreatic α-cells. The principal actions of glucagon involve regulation of metabolic pathways involved in glucose homeostasis1. Glucagon binding to the GCGR activates adenylyl cyclase through heterotrimeric Gs G proteins, leading to an increase in intracellular cAMP levels and subsequent activation of PKA signaling2. The glucagon receptor is a 485-amino acid protein with a central core consisting of seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal tail2.Glucagon receptors are widely expressed in multiple tissues including the liver, brain, pancreas, heart, kidney, and smooth muscle cell in the gastrointestinal tract and peripheral vasculature1.Abnormal glucagon signaling is associated with hyperglycemia in patients with type 2 diabetes3. Mice with homozygous null mutation of GCGR exhibit chronic hypoglycemia, extreme hyperglucagonemia, and α cell hyperplasia4. GCGR is a potential drug target for type 2 diabetes5.
Alternative names: Glucagon Receptor, GCGR -
基因ID
- 24953
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NCBI登录号
- NM_000160
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UniProt
- P30082
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途径
- Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
抗原
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