Gephyrin 抗体 (Intracellular)
Quick Overview for Gephyrin 抗体 (Intracellular) (ABIN7043214)
抗原
See all Gephyrin (GPHN) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 183-195, Intracellular
-
原理
- A Rabbit Polyclonal Antibody to Gephyrin
-
特异性
- Intracellular
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- Mouse,human - identical
-
产品特性
- Anti-Gephyrin Antibody is directed against an epitope of rat Gephyrin. Anti-Gephyrin Antibody (ABIN7043214, ABIN7044520 and ABIN7044521) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPHN from rat, mouse, and human samples.
-
纯化方法
- Affinity purified on immobilized antigen.
-
免疫原
-
Immunogen: Synthetic peptide
Immunogen Sequence: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin
-
亚型
- IgG
-
-
anti-Gephyrin (GPHN) antibody
GPHN 适用: 人, 猴 WB, IHC, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal 3B6 unconjugated
anti-Gephyrin (GPHN) antibodyGPHN 适用: 人, 大鼠, 猴 WB, FACS 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Gephyrin (GPHN) (AA 360-460) antibodyGPHN 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) antibodyGPHN 适用: 人, 小鼠, 大鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 677-769) antibodyGPHN 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 520-769) antibodyGPHN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 437-466) antibodyGPHN 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24625 unconjugated
anti-Gephyrin (GPHN) (AA 444-474), (Middle Region) antibodyGPHN 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 1-769) antibodyGPHN 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Gephyrin (GPHN) (AA 396-445) antibodyGPHN 适用: 人, 小鼠, 大鼠, 斑马鱼, 小鸡, 猴, Cow, 犬, 豚鼠, 马, 兔, 非洲爪蟾, Bat, Pig WB 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
-
说明
-
Negative Control: (ABIN7235588)
Blocking Peptide: (ABIN7235588)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- Gephyrin (GPHN)
-
别名
- GPHN
-
背景
-
GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase,Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.
Alternative names: Gephyrin, GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase -
基因ID
- 64845
-
NCBI登录号
- NM_020806
-
UniProt
- Q03555
-
途径
- Synaptic Membrane, Skeletal Muscle Fiber Development
抗原
-
