GFRA1 抗体 (Extracellular)
Quick Overview for GFRA1 抗体 (Extracellular) (ABIN7043211)
抗原
See all GFRA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 72-83, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GDNF Family Receptor α1
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特异性
- Extracellular domain
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-GFR alpha 1 (extracellular) Antibody is directed against an extracellular epitope of rat GDNF receptor α1. Anti-GFR alpha 1 (extracellular) Antibody (ABIN7043211, ABIN7044777 and ABIN7044778) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α1 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CRSAMEALKQKS, corresponding to amino acid residues 72-83 of rat GFRA1
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亚型
- IgG
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anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 239-350) antibody
GFRA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 25-280) antibodyGFRA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 351-429) antibodyGFRA1 适用: 人, 小鼠, 山羊 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 230-385) antibodyGFRA1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 32-119) antibodyGFRA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1G4 unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 32-119) antibodyGFRA1 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 4D8 unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 369-382) antibodyGFRA1 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) antibodyGFRA1 适用: 人 WB, ELISA, IF 宿主: 小鸡 Polyclonal unconjugated
anti-GDNF Family Receptor alpha 1 (GFRA1) (AA 25-227) antibodyGFRA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235577)
Blocking Peptide: (ABIN7235577)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
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别名
- GFRA1
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背景
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GFRA1, GFRα1, GDNF family receptor α1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1,The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3. In respect to their important role in development, individual knockout of either gdnf, gfra1 or ret gives rise to lethal phenotypes2.
Alternative names: GFR alpha 1, GFRA1, GFRalpha1, GDNF family receptor alpha1, GDNF receptor alpha 1, Ret ligand 1, RETL1, TRNR1 -
基因ID
- 25454
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NCBI登录号
- NM_145793
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UniProt
- Q62997
抗原
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