GIPR 抗体 (Extracellular)
Quick Overview for GIPR 抗体 (Extracellular) (ABIN7043209)
抗原
See all GIPR 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 25-38, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Gastric Inhibitory Polypeptide Receptor
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特异性
- Extracellular, N-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- 14 amino acid residues identical,Mouse - 13, human - 12
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产品特性
- Anti-GIPR (extracellular) Antibody (ABIN7043209, ABIN7044412 and ABIN7044413) is a highly specific antibody directed against an epitope of the rat Gastric inhibitory polypeptide receptor. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize GIPR from rat, human, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EGQTTGELYQRWER,corresponding to amino acid residues 25-38 of rat GIPR
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亚型
- IgG
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anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (AA 22-138) antibody
GIPR 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (Internal Region) antibodyGIPR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (AA 7-38), (N-Term) antibodyGIPR 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB18125 unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (Cytoplasmic Domain) antibodyGIPR 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (N-Term) antibodyGIPR 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (N-Term) antibodyGIPR 适用: 人, Cow, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) antibodyGIPR 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) antibodyGIPR 适用: 人 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) (AA 21-120) antibodyGIPR 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Gastric Inhibitory Polypeptide Receptor (GIPR) antibodyGIPR 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235569)
Blocking Peptide: (ABIN7235569)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GIPR (Gastric Inhibitory Polypeptide Receptor (GIPR))
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别名
- GIPR
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背景
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Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor,Gastric inhibitory polypeptide (GIP) is a 42-amino acid hormone released from the gastrointestinal tract and was first found to inhibit the secretion of hydrochloric acid from stomach parietal cells. GIP also stimulates postprandial insulin secretion from pancreatic β-cells following a pathway activated by the binding of GIP to its specific receptor thus making GIP, along with GLP-1, a major potentiator of glucose induced insulin secretion1.The human GIP receptor is a 466 amino-acid G-coupled protein receptor that is 81.5 % and 81.2 % identical to the hamster and rat receptors comprising seven potential transmembrane domains. This property is shared with glucagon and secretin receptor families.GIP has an affinity of Kd~0.2 nM to its receptor. GIPR activates several intracellular pathways including MAP kinase, phospholipase A2 but primarily activates adenylyl cyclase causing an increase in cAMP. GIP has been also found to be expressed outside the gut in organs such as the heart, brain and adipose tissue.In patients with Type 2 Diabetes-Mellitus, GIP levels are normal or at most minimally reduced therefore suggesting that there is a resistance of β-cells to GIP caused by a change in receptor expression signaling or mutation. In addition, mice whose GIPR gene expression is disrupted, following three months of high fat feeding, show reduced weight gain and adipose tissue compared to regular mice suggesting the elimination of this receptor may protect against the onset of severe obesity2.
Alternative names: GIPR, Gastric inhibitory polypeptide receptor, GIP receptor, Glucose-dependent insulinotropic polypeptide receptor -
基因ID
- 25024
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NCBI登录号
- NM_000164
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UniProt
- P43219
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途径
- Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
抗原
-
