GABRA4 抗体 (Extracellular)
Quick Overview for GABRA4 抗体 (Extracellular) (ABIN7043188)
抗原
See all GABRA4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 37-50, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GABA(A) α4 Receptor
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- 14 amino acid residues identical,Mouse, human - 10,rat - 13
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产品特性
- Anti-GABA(A) alpha4 Receptor (extracellular) Antibody is directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) Antibody (ABIN7043188, ABIN7044305 and ABIN7044306) can be used in western blot, immunohistochemistry and live cell imaging applications. It was designed to recognize GABRA4 from human, rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine
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亚型
- IgG
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anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (AA 36-258) antibody
GABRA4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) antibodyGABRA4 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (AA 401-500) antibodyGABRA4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6A12 unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (N-Term) antibodyGABRA4 适用: 人, 大鼠, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (Internal Region) antibodyVerified GABRA4 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (AA 350-377) antibodyGABRA4 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (Internal Region) antibodyGABRA4 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (N-Term) antibodyGABRA4 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (AA 333-361) antibodyGABRA4 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB31338 unconjugated
anti-gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4) (AA 350-377) antibodyGABRA4 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23935 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: ICC
Negative Control: (ABIN7235496)
Blocking Peptide: (ABIN7235496)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))
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别名
- GABRA4
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背景
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γ-Aminobutyric acid receptor subunit α4, GABRA4,GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic GABAB receptors (GABABR).GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ?1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.
Alternative names: GABA(A) α4 Receptor, gamma-Aminobutyric acid receptor subunit alpha4, GABRA4 -
基因ID
- 140675
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NCBI登录号
- NM_000809
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UniProt
- P28471
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途径
- Synaptic Membrane
抗原
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