GPR81 抗体 (Intracellular)
Quick Overview for GPR81 抗体 (Intracellular) (ABIN7043173)
抗原
See all GPR81 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 319-332, Intracellular
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原理
- A Rabbit Polyclonal Antibody to GPR81
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特异性
- Intracellular, C-terminus
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- The antibody will not recognize human GPR81 (HCAR1).
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预测反应
- Rat - 9,14 amino acid residues identical
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产品特性
- Anti-GPR81/HCAR1 Antibody (ABIN7043173, ABIN7044514 and ABIN7044515) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1)
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亚型
- IgG
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anti-G Protein-Coupled Receptor 81 (GPR81) (Cytoplasmic Domain) antibody
GPR81 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 81 (GPR81) (Extracellular Domain) antibodyGPR81 适用: 人, 大鼠, 猴, 犬, Bat, Hamster, 兔 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 81 (GPR81) (C-Term) antibodyGPR81 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 81 (GPR81) (Internal Region) antibodyVerified GPR81 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235445)
Blocking Peptide: (ABIN7235445)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR81 (G Protein-Coupled Receptor 81 (GPR81))
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别名
- HCAR1
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背景
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Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1,GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.
Alternative names: Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1 -
基因ID
- 243270
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NCBI登录号
- NM_032554
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UniProt
- Q8C131
抗原
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