GPR65 抗体 (Extracellular) (PE)
Quick Overview for GPR65 抗体 (Extracellular) (PE) (ABIN7043169)
抗原
See all GPR65 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 72-84, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR65 Conjugated to the Fluorescent Dye Phycoerythrin
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特异性
- 1st extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- rat - 11,Mouse - 12,13 amino acid residues identical
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产品特性
- Anti-GPR65 (TDAG8) (extracellular) Antibody (ABIN7043167, ABIN7044427 and ABIN7044428) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize GPR65 from rat, mouse, and human samples. \nAnti-GPR65 (TDAG8) (extracellular)-PE Antibody (ABIN7043169, ABIN7045545, ABIN7045546 and ABIN7045547) is directly conjugated to Phycoerythrin (PE). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DYTWNKDNWTFSP, corresponding to amino acid residues 72 - 84 of human GPR65
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亚型
- IgG
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anti-G Protein-Coupled Receptor 65 (GPR65) (AA 72-84), (Extracellular) antibody
GPR65 适用: 人 IHC, FACS, LCI, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (Extracellular Domain) antibodyGPR65 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) antibodyGPR65 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (AA 72-84), (Extracellular) antibody (FITC)GPR65 适用: 人 FACS, LCI 宿主: 兔 Polyclonal FITC
anti-G Protein-Coupled Receptor 65 (GPR65) (C-Term) antibodyGPR65 适用: 人, 小鼠, 犬, 马, Pig, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (3rd Extracellular Domain) antibodyGPR65 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (3rd Cytoplasmic Domain) antibodyGPR65 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 65 (GPR65) (1st Cytoplasmic Domain) antibodyGPR65 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7582046)
Blocking Peptide: (ABIN7235434)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR65 (G Protein-Coupled Receptor 65 (GPR65))
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别名
- GPR65
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背景
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G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor,GPR65, also called TDAG8 (T-cell death associated gene 8 protein), is a pH -sensing G-protein coupled receptor (GPCR) activated by acidic extracellular pH through the protonation of several histidine residues in the receptor's sequence. GPR65 is involved in cancer cell metastasis and proliferation, immune cell function, inflammation, and blood vessel formation1.Human GPR65 gene has been mapped to a location that is associated with T cell lymphoma and leukemia abnormalities.GPR65 is primarily expressed in immune cells and leukocyte-rich tissues such as circulating peripheral leukocytes, spleen, thymus, and tonsils. Expression is also detected pain relevant loci such as the dorsal root ganglia neurons and particularly small diameter neurons responsible for nociception1,2.GPR65 has the ability to affect tumor development and growth. Overexpression of GPR65 in Lewis lung carcinoma cells increases tumor growth in mice and may facilitate resistance to acidosis-mediated cell death in vitro through protein kinase A (PKA) and ERK related pathways1.Knockdown of GPR65 in NCI-H460 human non-small cell lung cancer cells decreases cell survival in acidic condition. Another study shows that activation of GPR65 by acidosis can promote evasion of cell apoptosis under glutamine starvation and its overexpression has been reported to transform immortalized mammary epithelial cells1.
Alternative names: G-protein coupled receptor 65, T-cell death-associated gene 8 protein, Psychosine receptor -
基因ID
- 8477
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NCBI登录号
- NM_003608
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UniProt
- Q8IYL9
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途径
- cAMP Metabolic Process
抗原
-
