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GPR55 抗体 (Extracellular) (FITC)

GPR55 适用: 小鼠 FACS, LCI 宿主: 兔 Polyclonal FITC
产品编号 ABIN7043164
发货至: 中国
  • 抗原 See all GPR55 抗体
    GPR55 (G Protein-Coupled Receptor 55 (GPR55))
    抗原表位
    • 16
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 261-275, Extracellular
    适用
    • 45
    • 8
    • 7
    • 3
    • 3
    • 1
    小鼠
    宿主
    • 47
    克隆类型
    • 47
    多克隆
    标记
    • 18
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPR55 antibody is conjugated to FITC
    应用范围
    • 29
    • 17
    • 9
    • 8
    • 4
    • 2
    • 2
    Flow Cytometry (FACS), Live Cell Imaging (LCI)
    原理
    A Rabbit Polyclonal Antibody to GPR55 Conjugated to the Fluorescent Dye FITC
    特异性
    3rd extracellular loop
    交叉反应
    小鼠, 大鼠
    预测反应
    15 amino acid residues identical, Rat - 11,Mouse - 14
    产品特性
    Anti-GPR55 (extracellular) Antibody (ABIN7043162, ABIN7044202 and ABIN7044203)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the protein from human samples. \nAnti-GPR55 (extracellular)-FITC Antibody (ABIN7043162, ABIN7044202 and ABIN7044203)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry. The antibody will not recognize the protein from human samples.
    纯化方法
    Affinity purified on immobilized antigen.
    免疫原

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55

    亚型
    IgG
    Top Product
    Discover our top product GPR55 Primary Antibody
  • 应用备注

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: N/A

    说明

    Negative Control: RIC-001-F

    Blocking Peptide: BLP-CR062

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    50 μL double distilled water (DDW).
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • 抗原
    GPR55 (G Protein-Coupled Receptor 55 (GPR55))
    别名
    GPR55 (GPR55 产品)
    背景
    G-Protein Coupled Receptor 55,G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5 % identical to the canonic cannabinoid receptor CB1 and 14.4 % identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.

    Alternative names: G-Protein Coupled Receptor 55
    基因ID
    227326
    NCBI登录号
    NM_005683
    UniProt
    Q3UJF0
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