GPR55 抗体 (Extracellular)
Quick Overview for GPR55 抗体 (Extracellular) (ABIN7043162)
抗原
See all GPR55 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 261-275, Extracellular
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原理
- A Rabbit Polyclonal Antibody to GPR55
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特异性
- 3rd extracellular loop
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- Not recommended for use with human samples.
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预测反应
- rat - 11,15 amino acid residues identical,Mouse - 14
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产品特性
- Anti-GPR55 (extracellular) Antibody (ABIN7043162, ABIN7044202 and ABIN7044203) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human, rat, and mouse samples. The antibody will not recognize the human protein.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RNRFILD(S)RMKQGIS, corresponding to amino acid residues 261 - 275 of mouse GPR55
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亚型
- IgG
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anti-G Protein-Coupled Receptor 55 (GPR55) (AA 160-174), (Extracellular) antibody
GPR55 适用: 人 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (AA 141-240) antibodyGPR55 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (C-Term) antibodyGPR55 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) antibodyGPR55 适用: 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (Cytoplasmic Domain) antibodyGPR55 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (AA 210-319) antibodyGPR55 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (AA 141-240) antibody (AbBy Fluor® 488)GPR55 适用: 人 WB, FACS 宿主: 兔 Polyclonal AbBy Fluor® 488
anti-G Protein-Coupled Receptor 55 (GPR55) (AA 203-222) antibodyGPR55 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (C-Term) antibodyGPR55 适用: 人 IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 55 (GPR55) (AA 261-275), (Extracellular) antibody (FITC)GPR55 适用: 小鼠 FACS, LCI 宿主: 兔 Polyclonal FITC
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: N/A
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说明
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Negative Control: (ABIN7235427)
Blocking Peptide: (ABIN7235427)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- GPR55 (G Protein-Coupled Receptor 55 (GPR55))
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别名
- GPR55
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背景
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G-Protein Coupled Receptor 55,G protein coupled receptor 55 (GPR55) is a cannabinoid receptor activated by a number of endo-, phyto- and synthetic cannabinoid ligands such as THC, JWH015 and anandamide1-3.GPR55 is only 13.5 % identical to the canonic cannabinoid receptor CB1 and 14.4 % identical to CB2. Upon ligand binding, it initiates a signaling cascade quite distinct from those used by CB1 and CB2 that eventually leads to an increase in intracellular calcium levels. GPR55 belongs to the Class A GPCRs. It shares many similarities with rhodopsin and as all other G-protein coupled receptors in its basic structure of seven transmembrane domains, intracellular C- terminus and extracellular N-terminal extensions4. The protein is highly expressed in large dorsal root ganglion neurons and its mRNA is present in the brain and periphery1.GPR55 has a role in self-renewing adult neural stem cell development and in hippocampal neurogenesis3. Experiments with mice in which GPR55 has been inactivated reveal a role for this receptor in neuropathic and inflammatory pain as well as in bone physiology. Thus deciphering the pharmacology of this receptor could lead to new therapeutics in these fields5.
Alternative names: G-Protein Coupled Receptor 55 -
基因ID
- 227326
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NCBI登录号
- NM_005683
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UniProt
- Q3UJF0
抗原
-
