EPHA1 抗体 (Extracellular)
Quick Overview for EPHA1 抗体 (Extracellular) (ABIN7043132)
抗原
See all EPHA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 457-471, Extracellular
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原理
- A Rabbit Polyclonal Antibody to EphA1 Receptor
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特异性
- Extracellular, N-terminus
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,rat - identical
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产品特性
- Anti-EphA1 (extracellular) Antibody (ABIN7043132, ABIN7044243 and ABIN7044244) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize EphA1 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KKEPRQLELTWAGSR, corresponding to amino acid residues 457-471 of human EphA1
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亚型
- IgG
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anti-Ephrin Type A Receptor 1 (EPHA1) (AA 296-414) antibody
EPHA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (C-Term) antibodyEPHA1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) antibodyEPHA1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 5D2 unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 394-500) antibodyEPHA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 8D4 unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 963-976), (C-Term) antibodyEPHA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 599-842) antibodyEPHA1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 716-960) antibodyEPHA1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 913-943), (C-Term) antibodyEPHA1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB1578 unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) (AA 29-60), (N-Term) antibodyEPHA1 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB1576 unconjugated
anti-Ephrin Type A Receptor 1 (EPHA1) antibodyEPHA1 适用: 人 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235318)
Blocking Peptide: (ABIN7235318)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- EPHA1 (Ephrin Type A Receptor 1 (EPHA1))
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别名
- EPHA1
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背景
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Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1,Eph receptors are the largest family of receptor tyrosine kinases (RTKs). EphA receptors (EphA1-10) bind ephrinA ligands which are GPI-linked proteins and EphB receptors (EphB1-6) bind ephrinB ligands which are membrane protein with one transmembrane domain1,2. Within each subfamily, interactions between receptor and ligand are promiscuous. In addition, Eph receptors can also bind ephrins from the other class2. Forward and reverse signaling through Eph receptors is a unique characteristic to this RTK since ephrins are physically linked to the plasma membrane3.Structurally, Eph receptors contain an extracellular ligand-binding domain, a transmembrane domain and an intracellular C-terminal domain responsible for intracellular signaling1. Forward Eph receptor signaling involves autophosphorylation of the receptor via a tyrosine kinase domain, as well as phosphorylation of other proteins. Known effectors of the forward signaling include Src kinase and Ras/Rho GTPases2. Much less is known about the reverse signaling mediated by Eph receptors. Besides from acting independently, Eph receptors can also signal in concert with other receptors. For example, Eph receptors cooperate with FGF receptor, NMDA ligand-gated ion channel and chemokine G-protein coupled receptor2.Biological activities attributed to the Eph receptor-ephrin signaling module include establishing neuronal connections, mediating neuronal plasticity and repair following neuronal injury2,4. Eph receptors may also have a role in the immune system5.Eph receptors are expressed in the developing nervous system, and in the adult brain. It is also detected in the pancreas, intestine, bone and lymphocytes. In cancer cells, Eph receptors and ephrins are overexpressed2,6,7. They are also implicated in neurodegenerative disorders like Alzheimer's disease1.
Alternative names: EphA1, Ephrin type-A receptor 1, Eph receptor A1, EPH tyrosine kinase 1, Tyrosine-protein kinase receptor EPH, EPHT1 -
基因ID
- 2041
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NCBI登录号
- NM_005232
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UniProt
- P21709
抗原
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