NBC4 抗体 (Intracellular)
Quick Overview for NBC4 抗体 (Intracellular) (ABIN7043118)
抗原
See all NBC4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 11-23, Intracellular
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原理
- A Rabbit Polyclonal Antibody to NBC4 Transporter
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特异性
- Intracellular, N-terminus
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交叉反应 （详细）
- Not recommended for human and mouse samples.
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预测反应
- Rat only
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产品特性
- Anti-Rat SLC4A5 (NBC4) Antibody is directed against an epitope of rat NBCe2. Anti-Rat SLC4A5 (NBC4) Antibody (ABIN7043118, ABIN7044819 and ABIN7044820) can be used in western blot analysis. It has been designed to recognize NBCe2 from rat samples. The antibody is not recommended for mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KLEPTSYRRRHPE, corresponding to amino acid residues 11-23 of rat NBCe2
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亚型
- IgG
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anti-Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) (AA 1042-1121) antibody
NBC4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) (AA 1071-1137) antibodyNBC4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) (AA 148-197) antibodyNBC4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) (AA 899-948) antibodyNBC4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4) (N-Term) antibodyNBC4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:400
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- NBC4 (Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4))
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别名
- SLC4A5
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背景
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Electrogenic sodium bicarbonate cotransporter 4, NBCe2,The SLC4 transporter family, also known as the bicarbonate (HCO3-)-transporter family, includes the products of ten human genes (SLC4A1-5, A7-11). In mammals, the SLC4 membrane proteins are critical for several physiological processes, including the carriage of carbon dioxide (CO2) from the systemic capillaries to the pulmonary capillaries, the regulation of cell volume in multiple cell types, and the regulation of intracellular pH in nearly every cell of the body1.NBCe2 (NBC4) is an electrogenic sodium bicarbonate co-transporter encoded by SLC4A5. There are six NBCe2 isoform (SLC4A5A-F) reported2. NBCe2 is an integral membrane protein consisting of 14 transmembrane domains. NBCe2 has a long N-terminal hydrophilic domain and a much shorter C-terminal hydrophilic domain, both of which are intracellular3.NBCe2 is predominantly detected in the brain (highest in prefrontal cortex), epididymis, cardiac muscle, smooth muscle, kidney, and choroid plexus/apical1.Several studies identified SLC4A5 as a positional candidate for hypertension4,5. In mice, disruption of slc4a5 causes both arterial hypertension as well as metabolic acidosis (distal renal tubular acidosis)6. In addition, mice lacking NBCe2 have reduced choroid plexus secretion and reduced ventricle size. These combined issues change intracranial pressure, CSF electrolytes, and cause severe eye pathologies such as photoreceptor loss, ganglion cell loss, and retinal detachment7.
Alternative names: SLC4A5 (NBC4), Electrogenic sodium bicarbonate cotransporter 4, NBCe2 -
基因ID
- 297386
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NCBI登录号
- NM_001386136
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UniProt
- Q6RI88
抗原
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