DRD4 抗体 (3rd Intracellular Loop)
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Quick Overview for DRD4 抗体 (3rd Intracellular Loop) (ABIN7043110)
抗原
See all DRD4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 3rd Intracellular Loop, AA 215-228
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原理
- A Rabbit Polyclonal Antibody to D4 Dopamine Receptor
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 9,14 amino acid residues identical
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产品特性
- Anti-D4 Dopamine Receptor Antibody is directed against an epitope located at the 3rd intracellular loop of the rat D4 dopamine receptor. Anti-D4 Dopamine Receptor Antibody (ABIN7043110 and ABIN7044233) can be used in western blot analysis, and recognizes DRD4 from rat and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RRWEAARHTKLHSR, corresponding to amino acid residues 215-228 of rat DRD4
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亚型
- IgG
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anti-Dopamine Receptor D4 (DRD4) (AA 365-391) antibody
DRD4 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB22909 unconjugated
anti-Dopamine Receptor D4 (DRD4) (C-Term) antibodyDRD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) (AA 100-200) antibodyDRD4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) (C-Term) antibodyDRD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) (AA 365-391) antibodyDRD4 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal 625CT1-5-4 unconjugated
anti-Dopamine Receptor D4 (DRD4) (C-Term) antibodyDRD4 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) (C-Term) antibodyDRD4 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) antibodyDRD4 适用: 人, 小鼠 WB, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Dopamine Receptor D4 (DRD4) (AA 355-404) antibodyDRD4 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235246)
Blocking Peptide: (ABIN7235246)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- DRD4 (Dopamine Receptor D4 (DRD4))
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别名
- DRD4
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背景
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Synonyms: DRD4, D4DR, D(2C) dopamine receptor
Description: The dopamine neurotransmitter belongs to the catecholamines and can therefore be further converted into adrenaline and noreadrenaline. Dopamine has various physiological roles, including learning and memory, motor output and endocrine regulation. It does so by binding and activating dopamine receptors which belong to the G-protein coupled receptor superfamily (GPCR)1.The D4 dopamine receptor (DRD4) belongs to the D2-like family as do D2 and D3 dopamine receptors. Like all GPCRs, it has seven transmembrane spanning membrane regions. Structure-wise, members of the family share high homology in the transmembrane domains and lower homology in the extracellular N-terminal and the intracellular C-terminal domains. Notably, the coding region of the 3rd intracellular loop of DRD4 is known to undergo extensive polymorphism2.Like many GPCRs, each dopamine receptor subtype can react with more than one G-protein giving rise to different signaling possibilities3. Whereas D2-like dopamine receptors are generally considered to couple to Gi, and therefore inhibit adenylyl cyclase, the signaling through DRD4 is complicated due to the polymorphisms in the 3rd intracellular loop. It seems that this region is important to G-coupling as different polyphormisms in the region influence the ability of DRD4 to couple to adenylyl cyclase and G-proteins4,5. D4 dopamine receptors also influence Ca2+ levels3,6. They could also interact with G-protein couple inwardly rectifying K+ channel to ultimately cause a decrease in the firing rate of neurons7.The distribution of DRD4 mostly includes the brain and is mainly found post-synaptically in dendritic shafts and spines of mammalian striatum8.
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基因ID
- 25432
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NCBI登录号
- NM_000797
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UniProt
- P30729
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途径
- cAMP Metabolic Process, Synaptic Membrane, Proton Transport, Photoperiodism, Negative Regulation of Transporter Activity
抗原
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