CFTR 抗体 (Cytosolic)
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北京 101111
Quick Overview for CFTR 抗体 (Cytosolic) (ABIN7043096)
抗原
See all CFTR 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 1468-1480, Cytosolic
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原理
- A Rabbit Polyclonal Antibody to Cystic Fibrosis Transmembrane Conductance Regulator
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特异性
- Cytoplasmic, C-terminal part
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,rat,pig - 13,14 amino acid residues identical
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产品特性
- Anti-CFTR Antibody (ABIN7043096, ABIN7044127 and ABIN7044128) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CFTR from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KEETEEEVQDTRL, corresponding to amino acid residues 1468-1480 of human CFTR
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亚型
- IgG
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anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) antibody
CFTR 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 1161-1480) antibodyCFTR 适用: 人 IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 258-385) antibodyCFTR 适用: 人 IHC (f), FACS 宿主: 小鼠 Monoclonal CFTR-1643 unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (Ser737) antibodyCFTR 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (Ser246) antibodyCFTR 适用: 人, 小鼠, 大鼠 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) antibodyCFTR 适用: 人 ISt, IHC (f) 宿主: 小鼠 Monoclonal rCFTR-1342 unconjugated Recombinant Antibody
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 258-385) antibodyCFTR 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 258-385) antibodyCFTR 适用: 人 ISt, IHC (f) 宿主: 小鼠 Monoclonal CFTR-1785 unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) antibodyCFTR 适用: 人 ISt, IHC (f) 宿主: 小鼠 Monoclonal CFTR-1342 unconjugated
anti-Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR) (AA 258-385) antibodyCFTR 适用: 人 ISt, IHC (f) 宿主: 兔 Monoclonal CFTR-1775R unconjugated Recombinant Antibody
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IP|IHC|ICC|IFC
Negative Control: (ABIN7235200)
Blocking Peptide: (ABIN7235200)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))
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别名
- CFTR
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背景
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Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel,The cystic fibrosis transmembrane conductance regulator (CFTR) is the most dominant Cl- channel in several epithelial tissues, especially in lung and colon. Remarkably, CFTR is a member of the ATP-binding cassette (ABC) transporter superfamily that uses ATP hydrolyzation as the driving force for the translocation of a wide variety of substrates including sugars, amino acids, proteins and hydrophobic compounds, across cellular membranes. The CFTR is unique among ABC transporters in that it is a cAMP-regulated Cl- channel. It shares the superfamily topology of 12 transmembrane domains with two nucleotide-binding domains (NBDs) and a regulatory (R) domain in the large third intracytoplasmic loop that is phosphorylated in multiple sites by PKA. Mutations in the CFTR gene cause channel dysfunction in several ways, ranging from complete loss of surface expression to diminished Cl- secretion. Defects in the CFTR gene cause cystic fibrosis (CF), the most common genetic disease among Caucasians, as well as a form of male sterility.Regulation of the CFTR channel is accomplished through the activation of surface receptors that couple to adenyl cyclase, raise cAMP cellular levels and thus activate PKA. This has been demonstrated for the adenosine and ß2 adrenergic receptor and the vasopressin hormone among others.Besides enhanced Cl- conductance, activation of CFTR also leads to the regulation of other ion channels. The best-studied case is its interaction with the epithelial Na+ channels (ENaC), although it can probably regulate other ion channels as well (Kir1.1 for example). The mechanism by which CFTR regulates other ion channels is not clear, but it may involve protein-protein interactions via molecules that interact with its C-terminal PDZ binding motif, such as the NHERF adaptor protein.
Alternative names: CFTR, Cystic fibrosis transmembrane conductance regulator, ATP-binding cassette sub-family C member 7, ABCC7, cAMP-dependent Cl- channel -
基因ID
- 1080
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NCBI登录号
- NM_000492
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UniProt
- P13569
抗原
-
