CNIH2, CNIH3 (AA 49-61), (Extracellular) 抗体
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Quick Overview for CNIH2, CNIH3 (AA 49-61), (Extracellular) 抗体 (ABIN7043072)
抗原
适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 49-61, Extracellular
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原理
- A Rabbit Polyclonal Antibody to CNIH2 and CNIH3
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特异性
- Extracellular loop
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交叉反应
- 小鼠, 大鼠
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交叉反应 （详细）
- This antibody will recognize both CNIH2 and CNIH3, but not the related proteins CNIH1 and CNIH4.
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预测反应
- CNIH2 = Mouse,human identicalCNIH3 = Rat,mouse,human - 11,13 amino acid residues identical
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产品特性
- Anti-CNIH2/3 (extracellular) Antibody (ABIN7043072, ABIN7044388 and ABIN7044389) is a highly specific antibody directed against a conserved sequence of the rat CNIH2 and CNIH3 proteins. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the proteins in living cells. It has been designed to recognize CNIH2 and CNIH3 from human, rat, and mouse samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: RARERLKNIERIC, corresponding to amino acid residues 49-61 of rat CNIH2
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亚型
- IgG
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anti-CNIH2, CNIH3 (AA 25-120) antibody
适用: 大鼠 WB, IF (cc), IF (p), ELISA, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235145)
Blocking Peptide: (ABIN7235145)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CNIH2, CNIH3
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背景
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Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3,Cornichon homologs (CNIHs) are a family of AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Cornichon 2 and 3 (CNIH2/3) are responsible for controlling the export of AMPARs from the endoplasmic reticulum and associate with synaptic AMPARs to modulate channel kinetics1,2.CNIHs are small proteins with three transmembrane domains that directly binds to AMPARs. Overexpression CNIHs and AMPA receptors causes their localization to the Golgi apparatus3.CNIH and TARP both compete for the binding to AMPAR subunits GluA2, GluA3, and GluA4 subunits suggesting both proteins bind to the same site. CNIH2/3 binding to AMPARs is dependent on AMPAR subunit composition and TARPs3,4.CNIH2/3 knock out mice shows alterations in synaptic content of GluA1-containing AMPARs, resulting in a reduction in AMPAR-mediated transmission and long-term potentiation2.
Alternative names: CNIH2/3, Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3 -
基因ID
- 361705, 690252
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NCBI登录号
- NM_182553
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UniProt
- Q5BJU5, D0Q0Y7
抗原
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