CNTF Receptor alpha 抗体 (Extracellular)
Quick Overview for CNTF Receptor alpha 抗体 (Extracellular) (ABIN7043071)
抗原
See all CNTF Receptor alpha (CNTFR) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 164-178, Extracellular
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原理
- A Rabbit Polyclonal Antibody to CNTF Receptor α
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特异性
- Extracellular
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse,human - identical
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产品特性
- Anti-CNTFRα (extracellular) Antibody (ABIN7043071 and ABIN7044187) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize CNTF receptor α from human, mouse, and rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRalpha
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亚型
- IgG
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anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 106-342) antibody
CNTFR 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 190-340) antibodyCNTFR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 151-160) antibodyCNTFR 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 120-358) antibodyCNTFR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 47-158) antibodyCNTFR 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 295-344) antibodyCNTFR 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (Internal Region) antibodyCNTFR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (AA 23-342) antibodyCNTFR 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (Internal Region) antibodyVerified CNTFR 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ciliary Neurotrophic Factor Receptor (CNTFR) (C-Term) antibodyCNTFR 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235141)
Blocking Peptide: (ABIN7235141)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CNTF Receptor alpha (CNTFR) (Ciliary Neurotrophic Factor Receptor (CNTFR))
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别名
- CNTFR
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背景
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Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha,CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRα)5.CNTFRα is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRα)6,7.CNTF binds to CNTFRα, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway7.CNTFRα knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRα has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRα and activates a similar signaling pathway to that of CNTF8.
Alternative names: CNTFRalpha, Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha -
基因ID
- 313173
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NCBI登录号
- NM_001207011
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UniProt
- Q08406
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途径
- JAK/STAT Signaling, Feeding Behaviour
抗原
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