CHRM4 抗体 (3rd Intracellular Loop)
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Quick Overview for CHRM4 抗体 (3rd Intracellular Loop) (ABIN7043064)
抗原
See all CHRM4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 3rd Intracellular Loop, AA 330-340
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原理
- A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 9,12 amino acid residues identical
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产品特性
- Anti-CHRM4 Antibody (ABIN7043064, ABIN7044584 and ABIN7044585) is a highly specific antibody directed against an epitope of the rat M4 muscarinic receptor. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize M4 from rat, mouse, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor
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亚型
- IgG
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anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 1-31) antibody
CHRM4 适用: 人 ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) antibodyCHRM4 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Cytoplasmic Domain) antibodyCHRM4 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, Hamster, 马, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 131-230) antibodyCHRM4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 201-236) antibodyCHRM4 适用: 人 WB 宿主: 兔 Polyclonal RB53327 unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Internal Region) antibodyCHRM4 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Internal Region) antibodyCHRM4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: 1:100
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235125)
Blocking Peptide: (ABIN7235125)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))
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别名
- CHRM4
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背景
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Synonyms: Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4
Description: Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily.Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut. mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10. M4 muscarinic receptor is mostly expressed in the striatum where it colocalizes with D1 dopamine receptors11.Malfunction of M4 muscarinic receptor is associated with a number of psychological and neurodegenerative diseases like Schizophrenia, Alzheimer's and Parkinson's diseases12.
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基因ID
- 25111
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NCBI登录号
- NM_000741
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UniProt
- P08485
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途径
- cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane
抗原
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