CHRM4 抗体 (AA 330-340)
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- 抗原 See all CHRM4 抗体
- CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))
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抗原表位
- AA 330-340
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CHRM4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)
- 原理
- A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M4
- 特异性
- 3rd intracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Mouse - identical, human - 9,12 amino acid residues identical
- 产品特性
- Anti-CHRM4 Antibody (ABIN7043064, ABIN7044584 and ABIN7044585)) is a highly specific antibody directed against an epitope of the rat M4 muscarinic receptor. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize M4 from rat, mouse, and human samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C) RTLNPASKWSK, corresponding to amino acid residues 330-340 of rat M4 muscarinic receptor
- 亚型
- IgG
- Top Product
- Discover our top product CHRM4 Primary Antibody
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anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 1-31) antibody
CHRM4 适用: 人 ELISA, FACS, IF (cc), IF (p), IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) antibodyCHRM4 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Cytoplasmic Domain) antibodyCHRM4 适用: 人, 大鼠, 小鼠, Cow, 豚鼠, Hamster, 马, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 131-230) antibodyCHRM4 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Internal Region) antibodyCHRM4 适用: 人, 大鼠, 小鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (AA 201-236) antibodyCHRM4 适用: 人 WB 宿主: 兔 Polyclonal RB53327 unconjugated
anti-Cholinergic Receptor, Muscarinic 4 (CHRM4) (Internal Region) antibodyCHRM4 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Negative Control: BLP-MR004
Blocking Peptide: BLP-MR004
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- CHRM4 (Cholinergic Receptor, Muscarinic 4 (CHRM4))
- 别名
- CHRM4 (CHRM4 产品)
- 背景
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Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4,Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily.Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut. mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10. M4 muscarinic receptor is mostly expressed in the striatum where it colocalizes with D1 dopamine receptors11.Malfunction of M4 muscarinic receptor is associated with a number of psychological and neurodegenerative diseases like Schizophrenia, Alzheimer's and Parkinson's diseases12.
Alternative names: M4 Muscarinic Receptor, CHRM4, Muscarinic acetylcholine receptor M4, Cholinergic receptor muscarinic 4, mAChR M4, HM4 - 基因ID
- 25111
- NCBI登录号
- NM_000741
- UniProt
- P08485
- 途径
- cAMP Metabolic Process, Inositol Metabolic Process, Synaptic Membrane
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