Muscarinic Acetylcholine Receptor M2 抗体 (AA 225-356)
Quick Overview for Muscarinic Acetylcholine Receptor M2 抗体 (AA 225-356) (ABIN7043062)
抗原
See all Muscarinic Acetylcholine Receptor M2 (CHRM2) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
-
-
抗原表位
- AA 225-356
-
原理
- A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M2
-
特异性
- 3rd intracellular loop
-
交叉反应
- 人, 小鼠, 大鼠
-
预测反应
- 132 amino acid residues identical, dog - 122, orangutan - 130, pig - 122,gorilla - identical,Chimpanzee, rat - 118, mouse - 117
-
产品特性
- Anti-CHRM2 Antibody (ABIN7043062, ABIN7044582 and ABIN7044583) is a highly specific antibody directed against an epitope of the human M2 muscarinic receptor. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize M2 from mouse, rat, and human samples.
-
纯化方法
- The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.
-
免疫原
-
Immunogen: GST fusion protein
Immunogen Sequence: GST fusion protein with the sequence VANQDPVSPSLVQGRIVKPN NNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSNDSTSV SAVASNMRDDEITQDENTVSTSLGHSKDENSKQTCIRIGTKTPKS DSCTPTNTTVEVVGSSGQNGDE, corresponding to amino acid residues 225-356 of human M2
-
亚型
- IgG
-
-
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) antibody
CHRM2 适用: 人, 小鼠 WB, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1424CT461-78-60 unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) antibodyCHRM2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 381-466) antibodyCHRM2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 208-388) antibodyCHRM2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (Cytoplasmic Domain) antibodyCHRM2 适用: 人, 小鼠, 大鼠, 猴, Cow, 犬, 豚鼠, Pig, 绵羊, 兔, 马, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (Cytoplasmic Domain) antibodyCHRM2 适用: 人, 小鼠, 大鼠, 猴, Cow, 犬, 豚鼠, Pig, 绵羊, 兔, Hamster, Bat IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (Cytoplasmic Domain) antibodyCHRM2 适用: 人 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 251-350) antibodyCHRM2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B5 unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 3-20) antibodyCHRM2 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 352-364) antibodyVerified CHRM2 适用: 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
-
-
应用备注
-
Antigen preadsorption control: 3 μg fusion protein per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
-
说明
-
Cited Application: IP|IHC|ICC
Negative Control: (ABIN7235118)
Blocking Peptide: (ABIN7235118)
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- 0.2 mL double distilled water (DDW).
-
浓度
- 1 mg/mL
-
缓冲液
- PBS pH 7.4
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
-
-
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
-
别名
- CHRM2
-
背景
-
Muscarinic acetylcholine receptor M2, Cholinergic receptor muscarinic 2, mAChR M2,The action of the neurotransmitter acetylcholine is mediated through two types of receptors, the ionotropic nicotinic receptors and the metabotropic muscarinic receptors. The muscarinic receptors belong to the superfamily of 7-transmembrane G-protein coupled receptors. Five subtypes of muscarinic receptors have been cloned and are named M1-M5.1-2The muscarinic receptors are widely distributed throughout the body but are predominantly expressed in the parasympathetic nervous system and exert both excitatory and inhibitory control over central and peripheral tissues.1-2Muscarinic receptors participate in a number of physiological functions such as regulation of heart rate, muscle contraction, cognition, sensory processing, and motor control.1 They also participate in learning and memory processing.3-4The M2 receptor is considered to be the predominant muscarinic receptor subtype that is expressed in cardiac muscle.5The M2 and M4 receptors mediate Ca2+ channel inhibition and Kir3 K+ channel activation by directly binding the Gβγ subunit to the channel.6,7 Stimulation of the M2 receptor by acetylcholine in the heart results in activation of the Kir3.1/Kir3.4 channels causing a slowing in heart beat.7
Alternative names: M2 Muscarinic Receptor, CHRM2, Muscarinic acetylcholine receptor M2, Cholinergic receptor muscarinic 2, mAChR M2 -
基因ID
- 1129
-
NCBI登录号
- NM_000739
-
UniProt
- P08172
抗原
-
