CLIC5 抗体 (Intracellular)
Quick Overview for CLIC5 抗体 (Intracellular) (ABIN7043058)
抗原
See all CLIC5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 161-174, Intracellular
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原理
- A Rabbit Polyclonal Antibody to CLIC5 Channel
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特异性
- Intracellular, C-terminus
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交叉反应
- 小鼠, 大鼠
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预测反应
- Mouse - identical, human - 9,14 amino acid residues identical
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产品特性
- Anti-CLIC5 Antibody (ABIN7043058, ABIN7044141 and ABIN7044142) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize CLIC5 from mouse, rat, and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DTNTHGDEKGSQRK, corresponding to amino acid residues 161-174 of rat CLIC5
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亚型
- IgG
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anti-Chloride Intracellular Channel 5 (CLIC5) (AA 91-190) antibody
CLIC5 适用: 人 WB, ELISA, IHC (p), IF 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (C-Term) antibodyCLIC5 适用: 人, 大鼠, 小鼠, 豚鼠, 马, 兔, Cow, 犬, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (C-Term) antibodyCLIC5 适用: 人, 豚鼠, 马, 兔, Cow, Pig, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (AA 230-279) antibodyCLIC5 适用: 人, 大鼠, 小鼠, 豚鼠, 马, 兔, 犬, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (AA 160-173) antibodyCLIC5 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal CLIC5-02 unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (AA 160-173) antibody (PE)CLIC5 适用: 人 ICFC 宿主: 小鼠 Monoclonal CLIC5-02 PE
anti-Chloride Intracellular Channel 5 (CLIC5) (Middle Region) antibodyCLIC5 适用: 人, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (C-Term) antibodyCLIC5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) (Middle Region) antibodyCLIC5 适用: 人, 大鼠, 小鼠, 豚鼠, 马, 兔, Cow, 犬, 斑马鱼, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Intracellular Channel 5 (CLIC5) antibodyCLIC5 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14724 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
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说明
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Negative Control: (ABIN7235110)
Blocking Peptide: (ABIN7235110)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CLIC5 (Chloride Intracellular Channel 5 (CLIC5))
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别名
- CLIC5
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背景
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Cl- intracellular channel 5,Chloride (Cl-) channels are membrane, anion-selective protein pores, which diffusively transport negative ions across their electrochemical gradient. Cl- channels are subdivided into six unique families, and their regulation mechanisms range from voltage-dependency, through G-protein activation, to mechanosensitivity, they may reside in either the plasma membrane or the membrane of intracellular compartments or both1. They are detected in the kidney, placenta, intestines, brain and the inner-ear1-4. The vertebrate Cl- intracellular channel (CLIC) family is composed of 6 highly conserved members (CLIC1-6), each exists as a ~250 residue protein which can assume both a soluble fold and a membrane-integral form. The latter consists of a single trans-membrane domain and is subsequently oligomerized to construct a functioning channel1,5. Interestingly, channels composed of CLIC4 and CLIC5 have been confirmed to be equally permeable to K+ and Cl-5.CLIC5, to which there are two isoforms - CLIC5A and CLIC5B - encoded by the same gene5, has first been isolated from human placenta6, and was shown, together with CLIC4 (with which it shares 76 % homology), to be enriched in the apical microvilli-containing part of the trophoblast epithelium, where, unlike CLIC4, it interacts with the actin cytoskeleton4. CLIC5 shares about 40 % homology with CLIC1-4 and is distributed to a high degree in myocytes, cardiomyocytes and stereocillia of the inner-ear2,4. Mice mutated in the Clic5 gene lack coordination and gradually become deaf2, show resistance to obesity, experience gastric ulcers and the resultant hemorrhage, and have higher incidence of entering torpor7.
Alternative names: CLIC5, Cl- intracellular channel 5 -
基因ID
- 94272
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NCBI登录号
- NM_001114086
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UniProt
- Q9EPT8
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途径
- Sensory Perception of Sound
抗原
-
