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- 抗原 See all CLCNKA 抗体
- CLCNKA (Chloride Channel Ka (CLCNKA))
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抗原表位
- AA 674-687, Intracellular
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CLCNKA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
- 原理
- A Rabbit Polyclonal Antibody to CLC-K Channel
- 特异性
- Intracellular, C-terminus
- 交叉反应
- 小鼠, 大鼠
- 预测反应
- Mouse - identical, human - 13,14 amino acid residues identical
- 产品特性
- Anti-CLC-K Antibody (ABIN7043054 and ABIN7044125) is an antibody directed against an epitope of rat CLC-K2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CLC-K channel from rat, mouse, and human samples. The antibody recognizes both CLC-K1 and CLC-K2 isoforms.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)KKAISTLTNPPAPK, corresponding to amino acid residues 674-687 of the rat longer form CLC-K2L
- 亚型
- IgG
- Top Product
- Discover our top product CLCNKA Primary Antibody
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anti-Chloride Channel Ka (CLCNKA) (C-Term) antibody
CLCNKA 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel Ka (CLCNKA) (C-Term) antibodyCLCNKA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel Ka (CLCNKA) antibodyCLCNKA 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel Ka (CLCNKA) (AA 470-644) antibodyCLCNKA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Chloride Channel Ka (CLCNKA) (N-Term) antibodyCLCNKA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- 说明
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Cited Application: IP|IHC
Negative Control: (ABIN7235094)
Blocking Peptide: (ABIN7235094)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- CLCNKA (Chloride Channel Ka (CLCNKA))
- 别名
- CLCNKA (CLCNKA 产品)
- 背景
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CLC-K1, CLC-K2, CLC-Ka, CLC-Kb, Clcnka, Clcnkb, ClCk, Chloride channel K,CLC-Ka and CLC-Kb are members of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. The human CLC-Ka and CLC-Kb (known as CLC-K1 and CLC-K2 in the rat) channels are closely related genes that share 94 % sequence homology and identical genomic organization.CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2, CLC-Ka and CLC-Kb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that CLC-4 and CLC-5 (and by inference CLC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved,the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-K channels require the presence of the auxiliary b subunit barttin, a 34 kD transmembrane protein, for transport to the plama membrane and regulation of channel permeation and gating.3CLC-K channels are expressed primarily in the kidney from the thin ascending limb to the collecting duct of the nephron, and in the stria vascularis and dark cells of the vestibular organ of the inner ear.The channels are important for renal salt reabsorption and water balance by enabling chloride exit across the basolateral membranes. The importance of the CLC-K channel in renal function is demonstrated by the fact that loss-of-function mutations in CLC-Kb lead to Bartter syndrome type III, an autosomal recessive disorder characterized by severe salt wasting, low blood pressure, hypokalemia and hypercalciuria.4
Alternative names: CLC-K, CLC-K1, CLC-K2, CLC-Ka, CLC-Kb, Clcnka, Clcnkb, ClCk, Chloride channel K - 基因ID
- 79430
- NCBI登录号
- NM_001042704
- UniProt
- P51802
- 途径
- Response to Water Deprivation
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