CDNF 抗体 (Mature)
Quick Overview for CDNF 抗体 (Mature) (ABIN7043031)
抗原
See all CDNF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 129-141, Mature
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原理
- A Rabbit Polyclonal Antibody to Cerebral Dopamine Neurotrophic Factor
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特异性
- Mature CDNF protein
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交叉反应
- 人, 小鼠
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交叉反应 （详细）
- Not recommended for rat samples.
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预测反应
- Mouse - 10,13 amino acid residues identical
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产品特性
- Anti-CDNF Antibody is directed against an epitope of human CDNF. Anti-CDNF Antibody (ABIN7043031, ABIN7044785 and ABIN7044786) can be used in western blot and immunohistochemistry applications. It has been designed to recognize CDNF from mouse and human samples. The antibody will not recognize CDNF from rat samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EKTLDLASVDLRK, corresponding to amino acid residues 129-141 of human CDNF
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亚型
- IgG
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anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 33-187) antibody
CDNF 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal D2 unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 20-183) antibodyCDNF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) antibodyCDNF 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7D6 unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) antibodyCDNF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 31-80) antibodyCDNF 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 20-183) antibodyCDNF 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 33-187) antibodyCDNF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cerebral Dopamine Neurotrophic Factor (CDNF) (AA 25-187) antibodyCDNF 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:200-1:500
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说明
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Negative Control: (ABIN7235051)
Blocking Peptide: (ABIN7235051)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))
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别名
- CDNF
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背景
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Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1,Neurotropic factors (NTFs) comprise an important group of secreted proteins regulating the life cycle of neurons during development. NTFs prevent apoptosis and control the number of neurons innervating a target tissue. NTFs also regulate migration, differentiation and maturation of postmitotic neuronal precursors and promote the regeneration of neurons after injury.Cerebral dopamine neurotrophic factor (CDNF) is a member of a sub-family of NTF proteins. The premature form of the protein consists of 187 amino acids while the mature form contains 161 residues. CDNF has a saposin-like N-terminal domain and an unstructured carboxy C-terminal domain with an intradomain cysteine bridge in a CXXC motif. Notably, saposin-like proteins interact with lipids, suggesting that CDNF's N-terminal may also interact with lipids. The saposin-like domain of CDNF crystalizes as a dimer at pH 4.6. It is presumed that CDNF functions upon synthesis and secretion since it does not contain prosequences for enzymatic activation.When CDNF is injected into the striatum of rats after the injection of 6-OHDA (6-OHDA leads to protracted retrograde degeneration of the nigrostriatal pathway) it significantly reduces amphetamine-induced rotational behavior and protects tyrosine hydroxylase (TH) positive dopaminergic cell bodies in the SN and TH-positive fibers in the striatum. In addition, CDNF had showed neurorestorative effects in the same animal model.Currently it is still unknown if CDNF also affects other types of neurons in the CNS or whether it has any effect on the PNS. It is also not clear to which target molecule CDNF binds and what signaling pathways it activates. CDNF might serve as a therapeutic agent for neurodegenerative diseases1.
Alternative names: CDNF, Cerebral dopamine neurotrophic factor, Arginine-rich mutated in early stage tumors-like 1, ARMET-like protein 1, ARMETL1 -
基因ID
- 441549
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NCBI登录号
- NM_001029954
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UniProt
- Q49AH0
抗原
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