CATSPER4 抗体 (1st Extracellular Loop)
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Quick Overview for CATSPER4 抗体 (1st Extracellular Loop) (ABIN7043028)
抗原
See all CATSPER4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 1st Extracellular Loop, AA 90-105
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原理
- A Rabbit Polyclonal Antibody to CatSper4 Channel
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat - identical, human -15,16 amino acid residues identical
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产品特性
- Anti-CatSper4 (extracellular) Antibody is directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (ABIN7043028, ABIN7044103 and ABIN7044104) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4
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亚型
- IgG
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anti-Cation Channel, Sperm Associated 4 (CATSPER4) (AA 306-472) antibody
CATSPER4 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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WB: 1:1000
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Cited Application: ICC
Negative Control: (ABIN7235039)
Blocking Peptide: (ABIN7235039)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))
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别名
- CATSPER4
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背景
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Synonyms: Cation channel sperm-associated protein 4
Description: During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence TxDxW indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH - and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.
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基因ID
- 329954
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NCBI登录号
- NM_198137
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UniProt
- Q8BVN3
抗原
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