CATSPER1 抗体 (3rd Extracellular Loop)
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Quick Overview for CATSPER1 抗体 (3rd Extracellular Loop) (ABIN7043027)
抗原
See all CATSPER1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- 3rd Extracellular Loop, AA 508-521
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原理
- A Rabbit Polyclonal Antibody to CatSper1 Channel
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交叉反应
- 小鼠, 大鼠
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预测反应
- Rat,human - 12,14 amino acid residues identical
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产品特性
- Anti-CatSper1 (extracellular) Antibody (ABIN7043027 and ABIN7044102) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CatSper1 in living cells. It has been designed to recognize CatSper1 from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
- (C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1
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亚型
- IgG
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anti-Cation Channel, Sperm Associated 1 (CATSPER1) antibody
CATSPER1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cation Channel, Sperm Associated 1 (CATSPER1) (N-Term) antibodyCATSPER1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cation Channel, Sperm Associated 1 (CATSPER1) (AA 36-65), (N-Term) antibodyCATSPER1 适用: 人 WB 宿主: 兔 Polyclonal RB32588 unconjugated
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应用备注
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WB: 1:200
FC: The optimal concentration should be determined by the user
ICC: The optimal concentration should be determined by the user
IHC: The optimal concentration should be determined by the user
IP: The optimal concentration should be determined by the user
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说明
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Negative Control: (ABIN7235036)
Blocking Peptide: (ABIN7235036)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.
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浓度
- 0.8 mg/mL
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缓冲液
- PBS pH 7.4, 1 % BSA with 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
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- CATSPER1 (Cation Channel, Sperm Associated 1 (CATSPER1))
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别名
- CATSPER1
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背景
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Synonyms: Cation channel sperm-associated protein 1
Description: Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte1-3. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis)3,4. Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pH i1,2, which render the sperm chemotaxis-responsive and hyperactive6. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust1-3. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm1-7. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity7, and is chemotactically responsive to progesterone8. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperβ (2 TM domains), CatSperγ and CatSperδ (1 TM domain each)1, 2. While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits1,3.CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels, a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel2. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+)3. Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels6.
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基因ID
- 225865
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NCBI登录号
- NM_053054
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UniProt
- Q91ZR5
抗原
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