GPR77 抗体 (Extracellular)
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- 抗原 See all GPR77 抗体
- GPR77 (G Protein-Coupled Receptor 77 (GPR77))
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抗原表位
- AA 179-193, Extracellular
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GPR77 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (IHC)
- 原理
- A Rabbit Polyclonal antibody to C5aR2 (extracellular).
- 特异性
- Extracellular, 2nd loop.
- 交叉反应
- 小鼠, 大鼠
- 交叉反应 （详细）
- Will not recognize Human C5aR2.
- 预测反应
- Mouse - 14 out of 15 amino acid residues identical, Rat - 13 out of 15 amino acid residues identical Will not recognize Human C5aR2
- 产品特性
- A rabbit polyclonal antibody to C5aR2 (extracellular).
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2
- 亚型
- IgG
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anti-G Protein-Coupled Receptor 77 (GPR77) (AA 2-19) antibody
GPR77 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) (Extracellular Domain) antibodyGPR77 适用: 人, 猴 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) (AA 1-337) antibodyGPR77 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) (C-Term) antibodyGPR77 适用: 人 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) antibodyGPR77 适用: 人, Non-Human Primate WB, FACS 宿主: 小鼠 Monoclonal 1D9-M12 unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) antibody (PE)GPR77 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 1D9-M12 PE
anti-G Protein-Coupled Receptor 77 (GPR77) (C-Term) antibodyGPR77 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) antibodyGPR77 适用: 人 FACS 宿主: 兔 Chimeric unconjugated Fc fragment
anti-G Protein-Coupled Receptor 77 (GPR77) (AA 258-337) antibodyGPR77 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-G Protein-Coupled Receptor 77 (GPR77) antibodyGPR77 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300
Application Dilutions Western blot wb: 1:400
- 说明
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Negative Control: (ABIN7235442)
Blocking Peptide: (ABIN7235442)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- GPR77 (G Protein-Coupled Receptor 77 (GPR77))
- 别名
- C5AR2 (GPR77 产品)
- 背景
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Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77,The complement system is a vital component of the innate immune system that play an important role in the immune responses to infectious organisms and tissue damages 1. The activation of the complement system is carried out through a proteolytic enzymes cascade generating various peptides and fragments 2.One of the most important component of the complement system is a protein named complement protein C5 3. Upon proteolytic cleavage of C5 two peptides are generated, C5a and C5b 4. C5a is known to be the most potent chemotactic factor in cells and responsible for the directed migration of cells involved in the immune response to infectious organisms and tissue damages 5. There are two different C5a receptors, C5aR1 (C5aR, CD88) and C5aR2 (C5L2, GPR77). C5aR1 is a member of the G-protein coupled receptors (GPCRs), and known to mediate the vast majority of C5a biological activity 6. In contrast C5aR2 does not exhibit any functional coupling to G proteins and it exact mechanism of action remains controversial 7. Expression of C5aR2 can be found in various tissues such as, bone marrow, spleen, lung and in immune cells such as myeloid cells and T cells 3.
Alternative names: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77 - 基因ID
- 319430
- NCBI登录号
- NM_018485
- UniProt
- Q8BW93
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