BRS3 抗体 (Extracellular)
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- 抗原 See all BRS3 抗体
- BRS3 (Bombesin-Like Receptor 3 (BRS3))
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抗原表位
- AA 186-201, Extracellular
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This BRS3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)
- 原理
- A Rabbit Polyclonal Antibody to Bombesin Receptor 3
- 特异性
- 2nd extracellular loop
- 交叉反应
- 人, 小鼠, 大鼠
- 预测反应
- Rat,mouse - 13,16 amino acids residues identical
- 产品特性
- Anti-Bombesin Receptor 3 (extracellular) Antibody is directed against a well conserved epitope of the human BB3 receptor. Anti-Bombesin Receptor 3 (extracellular) Antibody (ABIN7042966 and ABIN7043938) can be used in western blot analysis, as well as immunohistochemical and flow cytometry applications, and will recognize BRS3 from rat and mouse samples.
- 纯化方法
- Affinity purified on immobilized antigen.
- 免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3
- 亚型
- IgG
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anti-Bombesin-Like Receptor 3 (BRS3) antibody
BRS3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (N-Term) antibodyBRS3 适用: 人, 猴 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (Cytoplasmic Domain) antibodyBRS3 适用: 人, 小鼠, 大鼠, 猴, Cow, 犬, 豚鼠, Hamster, 马, Bat, 兔, 绵羊 ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (Cytoplasmic Domain) antibodyBRS3 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, Hamster, 马 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (AA 290-399) antibodyBRS3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (AA 161-210) antibodyBRS3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Bombesin-Like Receptor 3 (BRS3) (Internal Region) antibodyBRS3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50
Application Dilutions Western blot wb: 1:200-1:500
- 说明
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Negative Control: (ABIN7234864)
Blocking Peptide: (ABIN7234864)
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- 0.2 mL double distilled water (DDW).
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- 抗原
- BRS3 (Bombesin-Like Receptor 3 (BRS3))
- 别名
- BRS3 (BRS3 产品)
- 背景
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BB3R, BRS3,Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP.BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate.4,5Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6
Alternative names: Bombesin Receptor 3, BB3, BRS3 - 基因ID
- 680
- NCBI登录号
- NM_001727
- UniProt
- P32247
- 途径
- Feeding Behaviour
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