Bestrophin 2 抗体 (Extracellular)
Quick Overview for Bestrophin 2 抗体 (Extracellular) (ABIN7042965)
抗原
See all Bestrophin 2 (BEST2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 259-270, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Bestrophin-2 Channel
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特异性
- 3rd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat - identical, human - 11,12 amino acid residues identical
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产品特性
- Anti-Bestrophin-2 (extracellular) Antibody is directed against an epitope located at the 3rd extracellular loop of mouse Bestrophin-2. Anti-Bestrophin-2 (extracellular) Antibody (ABIN7042965 and ABIN7043936) can be used in western blot and immunohistochemical applications, and has been designed to recognize bestrophin-2 channel from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2
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亚型
- IgG
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anti-Bestrophin 2 (BEST2) antibody
BEST2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 2 (BEST2) (Internal Region) antibodyBEST2 适用: 人, 小鼠, 大鼠, 猴, Cow, Hamster, Pig IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 2 (BEST2) (Internal Region) antibodyBEST2 适用: 人, 猴, 犬 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 2 (BEST2) antibodyBEST2 适用: 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 2 (BEST2) (AA 374-403), (C-Term) antibodyBEST2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB24342 unconjugated
anti-Bestrophin 2 (BEST2) (AA 381-411), (C-Term) antibodyBEST2 适用: 人 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: IHC
Negative Control: (ABIN7234860)
Blocking Peptide: (ABIN7234860)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Bestrophin 2 (BEST2)
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别名
- BEST2
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背景
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BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1,Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy1,2. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. However, the molecular identity and functional role of CaCC in the brain have not been well established6. Bestrophin-2 is prominently expressed in colon and testes. Recently, Bestrophin-2 has also been found to be expressed in olfactory sensory neurons (OSNs)7.Two different topologies for Bestrophin-1 have been proposed. The first structure proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. The same structural controversy applies to Bestrophin-2. Bestrophin-1 and Bestrophin-2 (as well as Bestrophin-4 by heterologous expression) are activated by intracellular Ca2+ 10-12. Ca2+-dependent activation of Bestrophin-2 was also demonstrated in Xenopus13.
Alternative names: Bestrophin-2, BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1 -
基因ID
- 212989
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NCBI登录号
- NM_017682
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UniProt
- Q8BGM5
抗原
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