Bestrophin 1 抗体 (Extracellular)
Quick Overview for Bestrophin 1 抗体 (Extracellular) (ABIN7042964)
抗原
See all Bestrophin 1 (BEST1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 259-270, Extracellular
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原理
- A Rabbit Polyclonal Antibody to Bestrophin-1 Channel
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特异性
- 3rd extracellular loop
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交叉反应
- 人, 小鼠, 大鼠
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预测反应
- Rat - 10, human - 9,12 amino acid residues identical
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产品特性
- Anti-Bestrophin-1 (extracellular) Antibody is directed against an extracellular epitope of mouse Bestrophin-1. Anti-Bestrophin-1 (extracellular) Antibody (ABIN7042964, ABIN7043934 and ABIN7043935) can be used in western blot, immunohistochemistry and indirect flow cytometry applications. It has been designed to recognize bestrophin-1 channel from mouse, rat and human samples.
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纯化方法
- Affinity purified on immobilized antigen.
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免疫原
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)NPNKDYPGHEMD, corresponding to amino acid residues 259-270 of mouse Bestrophin-1
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亚型
- IgG
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anti-Bestrophin 1 (BEST1) (AA 200-300) antibody
BEST1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) antibodyBEST1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) antibodyBEST1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) (AA 1-604) antibodyBEST1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) (AA 361-460) antibodyBEST1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C2 unconjugated
anti-Bestrophin 1 (BEST1) (AA 376-585) antibodyBEST1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) antibodyBEST1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Bestrophin 1 (BEST1) (AA 292-585) antibodyBEST1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100
Application Dilutions Western blot wb: 1:200
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说明
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Cited Application: ICC
Negative Control: (ABIN7234856)
Blocking Peptide: (ABIN7234856)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 0.2 mL double distilled water (DDW).
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存条件
- 4 °C,-20 °C
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储存方法
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Bestrophin 1 (BEST1)
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别名
- BEST1
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背景
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BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1,Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy (BVMD)1,2. BVMD is mainly electrophysiologically characterized by a decrease in the light peak and physiologically by the thinning of the retina layer which eventually leads to the loss of central vision3. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. Recently, Bestrophin-1 was shown to be functionally expressed in astrocytes in both primary cell culture and in situ6. Bestrophin-1 is also detected in retina, brain, spinal cord and testes7.Two different topologies for Bestrophin-1 have been proposed. The first, the preferred structure, proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. Bestrophin-1, along with its counterparts, is activated by intracellular Ca2+. A recent study demonstrated that Bestrophin-1 indeed binds Ca2+ and by mutating specific residues, showed which amino acid residues are essential for binding Ca2+ 10, providing additional evidence that Bestrophin-1 is activated by direct binding of Ca2+ to the channel11,12.Bestrophin-1 has been found to release glutamate from astrocytes and is located at microdomains near synapses13.
Alternative names: Bestrophin-1, BEST1, Vitelliform macular dystrophy protein 2, VMD2, BMD1 -
基因ID
- 24115
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NCBI登录号
- NM_004183
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UniProt
- O88870
抗原
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